Key Laboratory for Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, P. R. China.
Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, P. R. China.
Chem Commun (Camb). 2024 Jun 25;60(52):6667-6670. doi: 10.1039/d4cc01646g.
Herein, a universal nucleic acid analysis platform was constructed for sensitive and accurate detection of miRNA-155 and ctDNA using isothermal amplification-assisted CRISPR/Cas12a and a tetrahedral DNA nanostructure (TDN) supported sensing interface. Under the optimal experimental conditions, the prepared sensor achieved specific detection of miRNA-155 and ctDNA at as low as aM levels in 2.6 h. Furthermore, the platform was also successfully applied to human serum sample recovery experiments and cancer cell lysates, demonstrating outstanding reliability and accuracy. We firmly believe that this work provides a universal, sensitive, and practical tool for early clinical diagnosis.
在此，我们构建了一种通用的核酸分析平台，该平台使用等温扩增辅助的 CRISPR/Cas12a 和四面体 DNA 纳米结构（TDN）支持的传感界面，实现了对 miRNA-155 和 ctDNA 的灵敏、准确检测。在最佳实验条件下，该传感器在 2.6 小时内实现了低至 aM 水平的 miRNA-155 和 ctDNA 的特异性检测。此外，该平台还成功应用于人血清样本回收实验和癌细胞裂解物，表现出出色的可靠性和准确性。我们坚信，这项工作为早期临床诊断提供了一种通用、灵敏、实用的工具。
