Department of Infectious Diseases, The First College of Clinical Medical Science, China Three Gorges University & Yichang Central People's Hospital, Yichang, China.
Central Laboratory, The First College of Clinical Medical Science, China Three Gorges University & Yichang Central People's Hospital, Yichang, China.
Immun Inflamm Dis. 2024 Jun;12(6):e1309. doi: 10.1002/iid3.1309.
Astragaloside IV (AS-IV) is the most active monomer in the traditional Chinese herbal medicine Radix Astragali, which has a wide range of antiviral, anti-inflammatory, and antifibrosis pharmacological effects, and shows protective effects in acute lung injury.
This study utilized the immunofluorescence, flow cytometry, enzyme-linked immunosorbent assay, quantitative reverse transcription-polymerase chain reaction, western blot, and hematoxylin and eosin staining methods to investigate the mechanism of AS-IV in reducing viral pneumonia caused by influenza A virus in A549 cells and BALB/c mice.
The results showed that AS-IV suppressed reactive oxygen species production in influenza virus-infected A549 cells in a dose-dependent manner, and subsequently inhibited the activation of nucleotide-binding oligomerization domain-like receptor thermal protein domain associated protein 3 inflammasome and Caspase-1, decreased interleukin (IL) -1β and IL-18 secretion. In BALB/c mice infected with Poly (I:C), oral administration of AS-IV can significantly reduce Poly (I:C)-induced acute pneumonia and lung pathological injury.
AS-IV alleviates the inflammatory response induced by influenza virus in vitro and lung flammation and structural damage caused by poly (I:C) in vivo.
黄芪甲苷（AS-IV）是中药黄芪中最活跃的单体，具有广泛的抗病毒、抗炎和抗纤维化的药理作用，并在急性肺损伤中表现出保护作用。
本研究采用免疫荧光、流式细胞术、酶联免疫吸附试验、定量逆转录聚合酶链反应、western blot 和苏木精和伊红染色方法，研究 AS-IV 降低甲型流感病毒感染 A549 细胞和 BALB/c 小鼠病毒性肺炎的机制。
结果表明，AS-IV 呈剂量依赖性抑制流感病毒感染 A549 细胞中活性氧的产生，随后抑制核苷酸结合寡聚结构域样受体热蛋白结构域相关蛋白 3 炎性体和 Caspase-1 的激活，减少白细胞介素（IL）-1β和 IL-18 的分泌。在 Poly（I:C）感染的 BALB/c 小鼠中，AS-IV 的口服给药可显著减轻 Poly（I:C）诱导的急性肺炎和肺病理损伤。
AS-IV 减轻了体外流感病毒诱导的炎症反应以及体内 Poly（I:C）引起的肺部炎症和结构损伤。
