测量叶片释放的氨。

Measuring the Release of Ammonia from Leaves.

机构信息

Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Tsukuba, Japan.

出版信息

Methods Mol Biol. 2024;2792:131-142. doi: 10.1007/978-1-0716-3802-6_11.

DOI:10.1007/978-1-0716-3802-6_11

PMID:38861084

Abstract

Ammonia (NH) is released from the leaves to the atmosphere when atmospheric NH concentration is low; in contrast, when atmospheric NH concentration is high, NH in the atmosphere is absorbed by the leaves. Some previous studies have examined relationships of such NH gas exchange with photorespiration, because a NH production reaction is involved in the photorespiratory pathway. NH compensation point (χ) is known as a parameter that represents an NH emission potential of the leaves. Two main procedures for determining the χ: "gas exchange method" and "apoplast extraction method" are explained in this chapter.

摘要

当大气中 NH 浓度较低时，氨（NH）从叶片释放到大气中；相反，当大气中 NH 浓度较高时，大气中的 NH 被叶片吸收。一些先前的研究已经检查了这种 NH 气体交换与光呼吸的关系，因为 NH 生产反应涉及光呼吸途径。NH 补偿点（χ）被认为是代表叶片 NH 排放潜力的参数。本章介绍了两种确定 χ 的主要方法：“气体交换法”和“质外体提取法”。