Riezman H
Cell. 1985 Apr;40(4):1001-9. doi: 10.1016/0092-8674(85)90360-5.
Yeast cells have been shown to internalize lucifer yellow CH by endocytosis. Internalization of the fluorescent dye is time-, temperature-, and energy-dependent, it is not saturable, and the dye is accumulated in the vacuole. Some of the yeast secretory mutants that accumulate endoplasmic reticulum or Golgi bodies are defective for endocytosis at restrictive temperature, while others are not. All of the mutants that accumulate secretory vesicles are defective for endocytosis. These results suggest that efficient transport of proteins from the endoplasmic reticulum to the Golgi apparatus and from the Golgi to secretory vesicles is not necessary for endocytosis. In contrast, endocytosis may be obligatorily coupled with the latest steps of secretion.
已证明酵母细胞通过内吞作用摄取荧光素黄CH。荧光染料的摄取是时间、温度和能量依赖性的，它不饱和，并且染料积累在液泡中。一些积累内质网或高尔基体的酵母分泌突变体在限制温度下内吞作用有缺陷，而其他的则没有。所有积累分泌囊泡的突变体内吞作用都有缺陷。这些结果表明，蛋白质从内质网到高尔基体以及从高尔基体到分泌囊泡的有效运输对于内吞作用不是必需的。相反，内吞作用可能必然与分泌的最后步骤相关联。
