运用单克隆抗亚类抗体测定类风湿关节炎滑膜浆细胞的免疫球蛋白亚类分布。

Immunoglobulin subclass distribution of synovial plasma cells in rheumatoid arthritis determined by use of monoclonal anti-subclass antibodies.

作者信息

Haber P L, Kubagawa H, Koopman W J

出版信息

Clin Immunol Immunopathol. 1985 Jun;35(3):346-51. doi: 10.1016/0090-1229(85)90095-9.

Abstract

The distribution of IgG and IgA subclass plasma cells among dissociated synovial cells from 14 rheumatoid arthritis (RA) synovia was examined by immunofluorescence using mouse monoclonal anti-human subclass antibodies. Of the IgG plasma cells 81 +/- 9% were IgG1, 4 +/- 2% IgG2, 14 +/- 9% IgG3, and 0.9 +/- 0.6% IgG4. While IgG1 predominated in all 14 synovia (which is similar to what is seen in normal tissues), in 5/14 20% or greater of IgG plasma cells were IgG3, suggesting a selective increase in IgG3 production in the synovia of certain RA patients. Among IgA plasma cells 89 +/- 5% were IgA1 and 8 +/- 3% were IgA2. This distribution is similar to the distribution in normal serum.

摘要

使用小鼠单克隆抗人亚类抗体，通过免疫荧光法检测了来自14例类风湿性关节炎（RA）滑膜的解离滑膜细胞中IgG和IgA亚类浆细胞的分布。在IgG浆细胞中，81±9%为IgG1，4±2%为IgG2，14±9%为IgG3，0.9±0.6%为IgG4。虽然IgG1在所有14例滑膜中均占主导地位（这与正常组织中的情况相似），但在14例中有5例，20%或更多的IgG浆细胞为IgG3，这表明某些RA患者的滑膜中IgG3产生有选择性增加。在IgA浆细胞中，89±5%为IgA1，8±3%为IgA2。这种分布与正常血清中的分布相似。