源自类风湿性滑膜组织多克隆库的人单克隆类风湿因子：制备与特性分析
Human monoclonal rheumatoid factors derived from the polyclonal repertoire of rheumatoid synovial tissue: production and characterization.
作者信息
Randen I, Thompson K M, Natvig J B, Førre O, Waalen K
机构信息
Institute of Immunology and Rheumatology, Oslo, Norway.
出版信息
Clin Exp Immunol. 1989 Oct;78(1):13-8.
Abstract
A panel of 20 human monoclonal antibodies was produced from cells derived from the synovial tissue of two rheumatoid arthritis patients and one polyarticular juvenile rheumatoid arthritis patient. Fourteen IgM monoclonal antibodies were classical rheumatoid factors (RFs) with specificities restricted to IgG and included twelve kappa and two lambda proteins. Two of them were pan-specific for IgG and reacted with all four human subclasses. Nine showed the Ga specificity, i.e. they reacted with IgG1, IgG2 and IgG4 subclass proteins. Two showed the 'new Ga-related' specificity, i.e. they reacted with IgG1, IgG2 and IgG4 subclass proteins, and also with the Ge3m(st) allotype proteins. One RF demonstrated an anti-G3m(u) anti-allotypic specificity. Five IgM(lambda) monoclonal antibodies and one IgM(kappa) monoclonal antibody demonstrated polyreactivity against various antigens including DNA, human thyroglobulin, human serum albumin and tetanus toxoid.
摘要
从两名类风湿性关节炎患者和一名多关节幼年型类风湿性关节炎患者的滑膜组织来源的细胞中制备了一组20种人单克隆抗体。14种IgM单克隆抗体是典型的类风湿因子（RFs），其特异性仅限于IgG，包括12种κ蛋白和2种λ蛋白。其中两种对IgG具有泛特异性，可与所有四种人亚类发生反应。9种显示出Ga特异性，即它们与IgG1、IgG2和IgG4亚类蛋白发生反应。两种显示出“新的Ga相关”特异性，即它们与IgG1、IgG2和IgG4亚类蛋白发生反应，也与Ge3m（st）同种异型蛋白发生反应。一种RF表现出抗G3m（u）抗同种异型特异性。5种IgM（λ）单克隆抗体和1种IgM（κ）单克隆抗体表现出对包括DNA、人甲状腺球蛋白、人血清白蛋白和破伤风类毒素在内的各种抗原的多反应性。
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