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核心技术专利：CN118964589B侵权必究
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白藜芦醇-氨苄西林双药负载聚乙烯吡咯烷酮/聚乙烯醇仿生电纺纳米纤维富含胶原蛋白，可有效修复烧伤创面。

Resveratrol-Ampicillin Dual-Drug Loaded Polyvinylpyrrolidone/Polyvinyl Alcohol Biomimic Electrospun Nanofiber Enriched with Collagen for Efficient Burn Wound Repair.

机构信息

Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow, India.

Department of Chemical Engineering, IIT Kanpur, Kanpur, India.

出版信息

Int J Nanomedicine. 2024 Jun 7;19:5397-5418. doi: 10.2147/IJN.S464046. eCollection 2024.

DOI:10.2147/IJN.S464046
PMID:38863647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11164821/
Abstract

BACKGROUND: The healing of burn wounds is a complicated physiological process that involves several stages, including haemostasis, inflammation, proliferation, and remodelling to rebuild the skin and subcutaneous tissue integrity. Recent advancements in nanomaterials, especially nanofibers, have opened a new way for efficient healing of wounds due to burning or other injuries. METHODS: This study aims to develop and characterize collagen-decorated, bilayered electrospun nanofibrous mats composed of PVP and PVA loaded with Resveratrol (RSV) and Ampicillin (AMP) to accelerate burn wound healing and tissue repair. RESULTS: Nanofibers with smooth surfaces and web-like structures with diameters ranging from 200 to 400 nm were successfully produced by electrospinning. These fibres exhibited excellent in vitro properties, including the ability to absorb wound exudates and undergo biodegradation over a two-week period. Additionally, these nanofibers demonstrated sustained and controlled release of encapsulated Resveratrol (RSV) and Ampicillin (AMP) through in vitro release studies. The zone of inhibition (ZOI) of PVP-PVA-RSV-AMP nanofibers against ( and () was found 31±0.09 mm and 12±0.03, respectively, which was significantly higher as compared to positive control. Similarly, the biofilm study confirmed the significant reduction in the formation of biofilms in nanofiber-treated group against both and . X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analysis proved the encapsulation of RSV and AMP successfully into nanofibers and their compatibility. Haemolysis assay (%) showed no significant haemolysis (less than 5%) in nanofiber-treated groups, confirmed their cytocompatibility with red blood cells (RBCs). Cell viability assay and cell adhesion on HaCaT cells showed increased cell proliferation, indicating its biocompatibility as well as non-toxic properties. Results of the in-vivo experiments on a burn wound model demonstrated potential burn wound healing in rats confirmed by H&E-stained images and also improved the collagen synthesis in nanofibers-treated groups evidenced by Masson-trichrome staining. The ELISA assay clearly indicated the efficient downregulation of TNF-alpha and IL-6 inflammatory biomarkers after treatment with nanofibers on day 10. CONCLUSION: The RSV and AMP-loaded nanofiber mats, developed in this study, expedite burn wound healing through their multifaceted approach.

摘要

背景：烧伤创面的愈合是一个复杂的生理过程，涉及多个阶段，包括止血、炎症、增殖和重塑，以重建皮肤和皮下组织的完整性。纳米材料，尤其是纳米纤维的最新进展为高效治疗因烧伤或其他损伤引起的伤口提供了新途径。

方法：本研究旨在开发和表征胶原蛋白修饰的双层静电纺丝纳米纤维垫，由 PVP 和 PVA 组成，负载白藜芦醇（RSV）和氨苄西林（AMP），以加速烧伤创面愈合和组织修复。

结果：通过静电纺丝成功制备了具有光滑表面和网状结构的纳米纤维，直径范围为 200-400nm。这些纤维表现出优异的体外性能，包括吸收伤口渗出物和在两周内进行生物降解的能力。此外，通过体外释放研究，这些纳米纤维显示出对包封的白藜芦醇（RSV）和氨苄西林（AMP）的持续和控制释放。PVP-PVA-RSV-AMP 纳米纤维对 （ 和 （）的抑菌环（ZOI）分别为 31±0.09mm 和 12±0.03mm，明显高于阳性对照。同样，生物膜研究证实，与对照相比，纳米纤维处理组中生物膜的形成显著减少。X 射线衍射（XRD）和傅里叶变换红外光谱（FTIR）分析证明 RSV 和 AMP 成功地包封在纳米纤维中及其相容性。溶血试验（%）显示纳米纤维处理组无明显溶血（小于 5%），证实其与红细胞（RBC）的细胞相容性。细胞活力试验和 HaCaT 细胞黏附试验表明，细胞增殖增加，表明其具有生物相容性和非毒性。在烧伤创面模型的体内实验结果表明，纳米纤维处理组的大鼠烧伤创面愈合有潜在的改善，通过 H&E 染色图像证实，并且在纳米纤维处理组中通过 Masson 三色染色证实胶原蛋白合成增加。ELISA 检测表明，纳米纤维治疗后第 10 天，TNF-α和 IL-6 炎症生物标志物的下调效率明显提高。

结论：本研究中开发的负载 RSV 和 AMP 的纳米纤维垫通过其多方面的方法加速烧伤创面愈合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/675bcb394d6e/IJN-19-5397-g0014.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/5f7c9a2201b3/IJN-19-5397-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/11fc88065ce0/IJN-19-5397-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/b18e0c6dc612/IJN-19-5397-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/915097406e1c/IJN-19-5397-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/143ea8b9ea63/IJN-19-5397-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/a2e22c252409/IJN-19-5397-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/7b4e2f0100fb/IJN-19-5397-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/b6642cbfef71/IJN-19-5397-g0011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b432/11164821/675bcb394d6e/IJN-19-5397-g0014.jpg

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[3]
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Front Bioeng Biotechnol. 2023-10-31

[4]
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[5]
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[6]
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[7]
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