Central Laboratory and Department of Ultrasound, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, No. 32, West Second Section, First Ring Road, Chengdu, Sichuan, 610072, China.
Department of Oncology, Affiliated Hospital of Yangzhou University. No.368, Hanjiang Road, Hanjiang District, Yangzhou, Jiangsu Province, 225012, China.
Adv Sci (Weinh). 2024 Aug;11(30):e2403156. doi: 10.1002/advs.202403156. Epub 2024 Jun 12.
With the comprehensive understanding of microorganisms and the rapid advances of physiochemical engineering and bioengineering technologies, scientists are advancing rationally-engineered bacteria as emerging drugs for treating various diseases in clinical disease management. Engineered bacteria specifically refer to advanced physiochemical or genetic technologies in combination with cutting edge nanotechnology or physical technologies, which have been validated to play significant roles in lysing tumors, regulating immunity, influencing the metabolic pathways, etc. However, there has no specific reviews that concurrently cover physiochemically- and genetically-engineered bacteria and their derivatives yet, let alone their distinctive design principles and various functions and applications. Herein, the applications of physiochemically and genetically-engineered bacteria, and classify and discuss significant breakthroughs with an emphasis on their specific design principles and engineering methods objective to different specific uses and diseases beyond cancer is described. The combined strategies for developing in vivo biotherapeutic agents based on these physiochemically- and genetically-engineered bacteria or bacterial derivatives, and elucidated how they repress cancer and other diseases is also underlined. Additionally, the challenges faced by clinical translation and the future development directions are discussed. This review is expected to provide an overall impression on physiochemically- and genetically-engineered bacteria and enlighten more researchers.
随着对微生物的全面认识以及物理化学工程和生物工程技术的快速进步，科学家们正在将经过合理设计的细菌作为新兴药物推进到临床疾病管理中，用于治疗各种疾病。工程菌是指通过先进的物理化学或遗传技术与前沿纳米技术或物理技术相结合，并经过验证可在裂解肿瘤、调节免疫、影响代谢途径等方面发挥重要作用的细菌。然而，目前还没有专门针对物理化学和遗传工程菌及其衍生物的综述，更不用说它们独特的设计原则和各种功能及应用了。本文主要介绍了物理化学和遗传工程菌的应用，并对其进行了分类和讨论，重点介绍了其针对不同用途和疾病（不限于癌症）的特定设计原则和工程方法。文中还强调了基于这些物理化学和遗传工程菌或细菌衍生物开发体内生物治疗剂的联合策略，以及它们如何抑制癌症和其他疾病的机制。此外，还讨论了临床转化所面临的挑战和未来的发展方向。本文旨在为物理化学和遗传工程菌提供全面的概述，并为更多的研究人员提供启示。
