Shandong Provincial Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, 250014, China.
Dongying Institute, Shandong Normal University, No. 2 Kangyang Road, Dongying, 257000, China.
Plant Cell Rep. 2024 Jun 12;43(7):167. doi: 10.1007/s00299-024-03258-z.
63 L. bicolor WRKY genes were identified and their informatics was analyzed. The results suggested that the LbWRKY genes involved in the development and salt secretion of salt glands in L. bicolor. Salt stress, as a universal abiotic stress, severely inhibits the growth and development of plants. WRKY transcription factors play a vital role in plant growth and development, as well as in response to various stresses. Nevertheless, little is known of systematic genome-wide analysis of the WRKY genes in Limonium bicolor, a model recretohalophyte. In this study, 63 L. bicolor WRKY genes were identified (LbWRKY1-63), which were unevenly distributed across seven chromosomes and one scaffold. Based on the structural and phylogenetic characteristics, 63 LbWRKYs are divided into three main groups. Cis-elements in the LbWRKY promoters were related to growth and development, phytohormone responses, and stress responses. Colinearity analysis showed strong colinearity between LbWRKYs and GmWRKYs from soybean (Glycine max). Therefore, LbWRKY genes maybe have similar functions to GmWRKY genes. Expression analysis showed that 28 LbWRKY genes are highly expressed in roots, 9 in stems, 26 in leaves, and 12 in flowers and most LbWRKY genes responded to NaCl, ABA, and PEG6000. Silencing LbWRKY10 reduced salt gland density and salt secretion ability of leaves, and the salt tolerance of the species. Consistent with this, genes associated with salt gland development were markedly down-regulated in the LbWRKY10-silenced lines. Our findings suggested that the LbWRKY genes involved in the development and salt secretion of salt glands in L. bicolor. Our research provides new insights into the functions of the WRKY family in halophytes.
鉴定了 63 个双色补血草 WRKY 基因，并对其信息学进行了分析。结果表明，LbWRKY 基因参与了双色补血草盐腺的发育和盐分泌。盐胁迫作为一种普遍的非生物胁迫，严重抑制植物的生长和发育。WRKY 转录因子在植物的生长发育以及对各种胁迫的响应中起着至关重要的作用。然而，对于模式盐生植物双色补血草中的 WRKY 基因的系统全基因组分析知之甚少。在这项研究中，鉴定了 63 个双色补血草 WRKY 基因（LbWRKY1-63），它们不均匀地分布在 7 条染色体和 1 个支架上。根据结构和系统发育特征，63 个 LbWRKY 分为 3 个主要组。LbWRKY 启动子中的顺式元件与生长发育、植物激素响应和应激响应有关。LbWRKY 与大豆（Glycine max）中的 GmWRKY 之间的共线性分析表明存在很强的共线性。因此，LbWRKY 基因可能具有与 GmWRKY 基因相似的功能。表达分析表明，28 个 LbWRKY 基因在根中高表达，9 个在茎中，26 个在叶中，12 个在花中，大多数 LbWRKY 基因对 NaCl、ABA 和 PEG6000 有反应。沉默 LbWRKY10 降低了叶片盐腺密度和盐分泌能力，以及该物种的耐盐性。与此一致的是，在 LbWRKY10 沉默系中与盐腺发育相关的基因明显下调。我们的研究结果表明，LbWRKY 基因参与了双色补血草盐腺的发育和盐分泌。我们的研究为 WRKY 家族在盐生植物中的功能提供了新的见解。
