Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, P.O. Box 7, Nablus, Palestine.
Dr. Belal Abu Helal Veterinary Clinic, Tulkarm, P304, Palestine.
J Infect Dev Ctries. 2024 May 30;18(5):809-816. doi: 10.3855/jidc.18835.
The objective of the present study was to report, for the first time, the presence of canine parvovirus type 2c (CPV-2c) in domesticated dogs with acute gastroenteritis and to characterize the antigenic variants circulating in Palestine.
A veterinary clinical-based epidemiological study was carried out between December 2022 and April 2023. Fifty fecal samples were collected from dogs with gastroenteritis and screened for CPV-2 infection by polymerase chain reaction. The distribution of positive cases according to various epidemiological factors was studied. Partial sequencing of the viral protein 2 (VP2) gene was performed for the analysis of CPV-2 variants.
Most of the investigated samples (60%; n = 50) during the study period were found positive for CPV-2 infection. There was no difference in the distribution of positive cases of CPV-2 infection based on age group, gender, location, and vaccination status. The analysis of nucleotide and amino acid sequences from amplified products, as well as phylogenetic analysis, revealed the presence of CPV-2c clustered with Asian CPV-2c variants.
In summary, this study represents the initial genetic analysis of CPV-2 present in Palestinian dogs with gastroenteritis and provides evidence that confirms the existence of the CPV-2c variants. To determine the prevailing CPV-2 variant associated with the infection, it is crucial to conduct further sequence analysis using large populations of both domestic and wild canines.
本研究的目的是首次报告在患有急性胃肠炎的家养犬中存在犬细小病毒 2c 型（CPV-2c），并描述在巴勒斯坦流行的抗原变异株。
在 2022 年 12 月至 2023 年 4 月期间进行了一项基于兽医临床的流行病学研究。从患有胃肠炎的犬中采集了 50 份粪便样本，并通过聚合酶链反应（PCR）检测 CPV-2 感染。根据各种流行病学因素研究阳性病例的分布情况。对病毒蛋白 2（VP2）基因的部分序列进行了分析，以研究 CPV-2 变异株。
在研究期间，大多数（60%；n=50）调查样本被检测出 CPV-2 感染呈阳性。CPV-2 感染的阳性病例在年龄组、性别、地理位置和疫苗接种状态方面没有差异。对扩增产物的核苷酸和氨基酸序列分析以及系统发育分析表明，存在与亚洲 CPV-2c 变异株聚类的 CPV-2c。
总之，本研究代表了对巴勒斯坦患有胃肠炎的犬中 CPV-2 的初步遗传分析，并提供了证实 CPV-2c 变异株存在的证据。为了确定与感染相关的主要 CPV-2 变异株，需要使用大量的家养和野生动物进行进一步的序列分析。
