State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, PR China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Colloids Surf B Biointerfaces. 2024 Sep;241:114018. doi: 10.1016/j.colsurfb.2024.114018. Epub 2024 Jun 7.
The pressing need for highly efficient antibacterial strategies arises from the prevalence of microbial biofilm infections and the emergence of rapidly evolving antibiotic-resistant strains of pathogenic bacteria. Photodynamic therapy represents a highly efficient and compelling antibacterial approach, offering promising prospects for effective control of the development of bacterial resistance. However, the effectiveness of many photosensitizers is limited due to the reduced generation of reactive oxygen species (ROS) in hypoxic microenvironment, which commonly occur in pathological conditions such as inflammatory and bacteria-infected wounds. Herein, we designed and prepared two phenothiazine-derived photosensitizers (NB-1 and NB-2), which can effectively generate superoxide anion radicals (O) through the type I process. Both photosensitizers demonstrate significant efficacy in vitro for the eradication of broad-spectrum bacteria. Moreover, NB-2 possesses distinct advantages including strong membrane binding and strong generation of O, rendering it an exceptionally efficient antibacterial agent against mature biofilms. In addition, laser activated NB-2 could be applied to treat MRSA-infected wound in vivo, which offers new opportunities for potential practical applications.
高效抗菌策略的迫切需求源于微生物生物膜感染的普遍存在和致病性细菌对抗生素耐药性的快速演变。光动力疗法是一种高效且引人注目的抗菌方法，为有效控制细菌耐药性的发展提供了有前景的途径。然而，由于缺氧微环境中活性氧 (ROS) 的产生减少，许多光敏剂的有效性受到限制，这种缺氧微环境在炎症和细菌感染的伤口等病理条件下很常见。在此，我们设计并制备了两种吩噻嗪衍生的光敏剂（NB-1 和 NB-2），它们可以通过 I 型过程有效地产生超氧阴离子自由基（O）。这两种光敏剂在体外均表现出广谱杀菌的显著功效。此外，NB-2 具有较强的膜结合能力和 O 的产生能力，使其成为一种对抗成熟生物膜的高效抗菌剂。此外，激光激活的 NB-2 可用于治疗体内耐甲氧西林金黄色葡萄球菌 (MRSA) 感染的伤口，为实际应用提供了新的机会。
