Plant Genomics & Molecular Improvement of Colored Fiber Laboratory, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310008, China.
Sci Data. 2024 Jun 12;11(1):620. doi: 10.1038/s41597-024-03481-z.
Recent advancements in plant regeneration and synthetic polyploid creation have been documented in Gossypium arboreum ZB-1. These developments make ZB-1 a potential model within the Gossypium genus for investigating gene function and polyploidy. This work generated the sequence and annotation of the ZB-1 genome. The contig-level genome was constructed using the PacBio high-fidelity reads, encompassing 81 contigs with an N50 length of 112.12 Mb. The Hi-C data assisted the construction of the chromosome-level genome, which consists of 13 pseudo-chromosomes and 39 un-anchored contigs, with a total length of about 1.67 Gb. Repetitive sequences accounted for about 69.7% of the genome in length. Based on ab initio and evidence-based prediction, we have identified 48,021 protein-coding genes in the ZB-1 genome. Comparative genomics analysis revealed conserved gene content and arrangement between ZB-1 and G. arboreum SXY1. The single nucleotide polymorphism occurrence rate between ZB-1 and SXY1 was about 0.54 per 1,000 nucleotides. This study enriched the genomic resources for further exploration into cotton regeneration and polyploidy mechanisms.
最近在 Gossypium arboreum ZB-1 中记录了植物再生和合成多倍体创造的进展。这些发展使 ZB-1 成为研究基因功能和多倍体的 Gossypium 属中的潜在模型。这项工作生成了 ZB-1 基因组的序列和注释。使用 PacBio 高保真读长构建了基于 contig 的基因组，其中包含 81 个 contig，N50 长度为 112.12Mb。Hi-C 数据有助于构建染色体水平的基因组，该基因组由 13 个假染色体和 39 个未锚定的 contig 组成，总长度约为 1.67Gb。重复序列约占基因组长度的 69.7%。基于从头预测和基于证据的预测，我们在 ZB-1 基因组中鉴定了 48,021 个蛋白质编码基因。比较基因组学分析显示 ZB-1 和 G. arboreum SXY1 之间具有保守的基因内容和排列。ZB-1 和 SXY1 之间的单核苷酸多态性发生率约为每 1000 个核苷酸 0.54 个。这项研究丰富了基因组资源，以进一步探索棉花再生和多倍体机制。
