Aubin J E
J Dent Res. 1985 Apr;64 Spec No:515-22. doi: 10.1177/002203458506400404.
The use of specific polyclonal and monoclonal antibodies as probes to study odontoblast morphology, function, and differentiation has received relatively little attention. The extent of the odontoblast processes in human and rat teeth is one question that we have approached recently by utilizing antibodies specific for intracellular elements, i.e., the cytoskeleton. Indirect immunofluorescence on both paraffin-embedded thin sections and surface-demineralized collagenase-digested whole mounts has indicated that the odontoblast process does extend to the dentino-enamel junction. By using other antibodies which recognize intra- and extracellular components, or antibodies which recognize unique antigens expressed at the cell surface of the odontoblast or its precursor cells, many more precise molecular details of odontoblast form and function would become accessible for analysis.
使用特定的多克隆抗体和单克隆抗体作为探针来研究成牙本质细胞的形态、功能和分化，相对而言受到的关注较少。我们最近通过利用针对细胞内成分（即细胞骨架）的特异性抗体，来探讨人和大鼠牙齿中，成牙本质细胞突起的范围这一问题。对石蜡包埋的薄片以及经表面脱矿质胶原酶消化的整装片进行间接免疫荧光检测，结果表明成牙本质细胞突起确实延伸至牙本质-釉质界。通过使用其他识别细胞内和细胞外成分的抗体，或者识别在成牙本质细胞或其前体细胞表面表达的独特抗原的抗体，就可以获取更多关于成牙本质细胞形态和功能的精确分子细节，以供分析。
