Sögaard-Pedersen B, Boye H
Public Child Dental Health Service, Klemensker, Denmark.
Scand J Dent Res. 1990 Oct;98(5):374-9. doi: 10.1111/j.1600-0722.1990.tb00987.x.
The subplasmalemmal cytoskeleton in human odontoblasts was studied by high resolution scanning electron microscopy. The odontoblast layer was isolated and exposed to formaldehyde, glutaraldehyde, and OsO4 for some specimens, while the membraneous structures and soluble proteins in the dental tissue were removed by Zenker's solution and 1% OsO4 for other specimens, without further fixation of the remaining components. The cytoskeletal elements comprised a dense network of interlacing filaments of different diameters in the cell body. Most cytoskeletal elements were parallel to the axis of the cell processes situated inside the dentinal tubules. The appearance and orientation of the investigated subplasmalemmal cytoskeletal elements was unaffected by the choice of method. Both methods confirm the presence of a subplasmalemmal cytoskeleton in human odontoblasts.
采用高分辨率扫描电子显微镜对人成牙本质细胞的亚膜细胞骨架进行了研究。对于一些标本，分离出牙本质细胞层并将其暴露于甲醛、戊二醛和四氧化锇中；而对于其他标本，先用岑克尔氏液和1%的四氧化锇去除牙组织中的膜性结构和可溶性蛋白质，其余成分不再进一步固定。细胞骨架成分在细胞体内构成了一个由不同直径的交错细丝组成的致密网络。大多数细胞骨架成分与位于牙本质小管内的细胞突起的轴平行。所研究的亚膜细胞骨架成分的外观和取向不受方法选择的影响。两种方法均证实人成牙本质细胞中存在亚膜细胞骨架。
