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半融合隔膜中的瞬态孔。

Transient pores in hemifusion diaphragms.

机构信息

Institute for Theoretical Physics, Georg-August University, Göttingen, Germany.

Institute for Theoretical Physics, Georg-August University, Göttingen, Germany; Technische Universität Dortmund, Dortmund, Germany.

出版信息

Biophys J. 2024 Aug 20;123(16):2455-2475. doi: 10.1016/j.bpj.2024.06.009. Epub 2024 Jun 11.

DOI:10.1016/j.bpj.2024.06.009
PMID:38867448
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11365115/
Abstract

Exchange of material across two membranes, as in the case of synaptic neurotransmitter release from a vesicle, involves the formation and poration of a hemifusion diaphragm (HD). The nontrivial geometry of the HD leads to environment-dependent control, regarding the stability and dynamics of the pores required for this kind of exocytosis. This work combines particle simulations, field-based calculations, and phenomenological modeling to explore the factors influencing the stability, dynamics, and possible control mechanisms of pores in HDs. We find that pores preferentially form at the HD rim, and that their stability is sensitive to a number of factors, including the three line tensions, membrane tension, HD size, and the ability of lipids to "flip-flop" across leaflets. Along with a detailed analysis of these factors, we discuss ways that vesicles or cells may use them to open and close pores and thereby quickly and efficiently transport material.

摘要

两种膜之间的物质交换，如囊泡中突触神经递质的释放，涉及到半融合膈膜（HD）的形成和穿孔。HD 的非平凡几何形状导致了环境依赖性的控制，这涉及到这种胞吐作用所需的孔的稳定性和动力学。这项工作结合了粒子模拟、基于场的计算和唯象模型，以探索影响 HD 中孔的稳定性、动力学和可能的控制机制的因素。我们发现，孔优先在 HD 边缘形成，其稳定性对许多因素敏感，包括三个线张力、膜张力、HD 大小以及脂质跨叶翻转的能力。除了对这些因素进行详细分析外，我们还讨论了囊泡或细胞可能利用这些因素来打开和关闭孔，从而快速有效地运输物质的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/22614e19b47d/gr17.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/683e8abeb63a/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/16262d83ae37/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/8f5439dc7783/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/c76904c40dd8/gr14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/870e319a1ece/gr15.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/f4885b6b6c15/gr16.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/22614e19b47d/gr17.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/e4a8a7dcb59f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/180d49f59d96/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/4aa8b2da9096/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/4b3c020c6f81/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/5d7fb8e5331e/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/65235f92f8d0/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/acb887933e11/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/fe3da7d98b5a/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/c2df2b2ec08f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/6c649f37ccf3/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/683e8abeb63a/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/16262d83ae37/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/8f5439dc7783/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/c76904c40dd8/gr14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/870e319a1ece/gr15.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/f4885b6b6c15/gr16.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fffe/11365115/22614e19b47d/gr17.jpg

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