Liu Xiaoguang, Wang Huiguo, Zhu Lin
School of Sport and Health, Guangzhou Sport University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou Sport University, Guangzhou, China.
Heliyon. 2024 May 25;10(11):e31917. doi: 10.1016/j.heliyon.2024.e31917. eCollection 2024 Jun 15.
The mechanisms occur in children with obesity after lifestyle intervention remain poorly explained. Here, we investigated the serum proteomes and metabolomes of children with obesity who had undergone 30 days of weight loss intervention.
Serum samples and clinical parameters were collected before and after lifestyle alteration interventions. Proteomic and metabolomic profiling was used to identify the differentially expressed proteins and differentially abundant metabolites in response to weight loss intervention. Lifestyle alteration interventions significantly decreased BMI, waist circumference, hip circumference and body fat, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL) and high non-HDL cholesterol, but not TG and high-density lipoprotein cholesterol (HDL), in children with obesity. By comparing the multiomics data, we identified 43 proteins and 165 metabolites that were significantly differentially expressed in children with obesity before and after lifestyle alteration interventions. Using integrated -omics analysis, we obtained 7 KEGG pathways that were organically integrated based on the correlations between differentially expressed proteins (DEPs) and metabolites (DMs). Further interaction analysis identified 7 proteins as candidate DEPs and 9 metabolites as candidate DMs. Interestingly, we found that some of these candidate DEPs and candidate DMs were significantly correlated with clinical parameters.
Our results provide valuable proteome and metabolome data resources for better understanding weight loss-associated responses in children with obesity. In addition, we analyzed the number of significantly differentially expressed proteins and metabolites, shed new light on weight loss pathogenesis in children with obesity, and added potential therapeutic agents for obese children.
生活方式干预后肥胖儿童体内发生的机制仍未得到充分解释。在此，我们研究了接受30天减肥干预的肥胖儿童的血清蛋白质组和代谢组。
在生活方式改变干预前后收集血清样本和临床参数。蛋白质组学和代谢组学分析用于鉴定减肥干预后差异表达的蛋白质和差异丰富的代谢物。生活方式改变干预显著降低了肥胖儿童的体重指数、腰围、臀围和体脂、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL）和高非高密度脂蛋白胆固醇，但未降低TG和高密度脂蛋白胆固醇（HDL）。通过比较多组学数据，我们鉴定出43种蛋白质和165种代谢物在生活方式改变干预前后的肥胖儿童中显著差异表达。使用综合组学分析，我们获得了7条基于差异表达蛋白质（DEPs）和代谢物（DMs）之间的相关性而有机整合的KEGG通路。进一步的相互作用分析确定了7种蛋白质为候选DEPs，9种代谢物为候选DMs。有趣的是，我们发现其中一些候选DEPs和候选DMs与临床参数显著相关。
我们的结果为更好地理解肥胖儿童的减肥相关反应提供了有价值的蛋白质组和代谢组数据资源。此外，我们分析了显著差异表达的蛋白质和代谢物的数量，为肥胖儿童的减肥发病机制提供了新的线索，并增加了肥胖儿童的潜在治疗药物。
