Wang Mengjie, Zhang Bin, Jin Feng, Li Genhua, Cui Changmeng, Feng Song
Clinical College of Neurology, Neurosurgery and Neurorehabilitation, Tianjin Medical University, Tianjin, 300070, China.
Department of Neurosurgery, Affiliated Hospital of Jining Medical University, No.133, Lotus Road, Jining, Shandong, China.
Heliyon. 2024 May 28;10(11):e32022. doi: 10.1016/j.heliyon.2024.e32022. eCollection 2024 Jun 15.
Moyamoya disease currently lacks a suitable method for early clinical screening.This study aimed to identify a simple and feasible clinical screening index by investigating microRNAs carried by peripheral blood exosomes. Experimental subjects participated in venous blood collection, and exosomes were isolated using Exquick-related technology. Sequencing was performed on the extracted exosomal ribonucleic acids (RNAs) to identify differential microRNAs. Verification of the results involved selecting relevant samples from the genetic database. The study successfully pinpointed a potential marker for early screening, hsa-miR-328-3p + hsa-miR-200c-3p carried by peripheral blood exosomes. Enrichment analysis of target genes revealed associations with intercellular junctions, impaired cytoskeletal regulation, and increased fibroblast proliferation, leading to bilateral internal carotid artery neointimal expansion and progressive stenosis. These findings establish the diagnostic value of hsa-miR-328-3p+hsa-miR-200c-3p in screening moyamoya disease, while also contributing to a deeper understanding of its underlying pathophysiology. Significant differences in microRNA expressions derived from peripheral blood exosomes were observed between moyamoya disease patients and control subjects. Consequently, the utilization of peripheral blood exosomes, specifically hsa-miR-328-3p + hsa-miR-200c-3p, holds potential for diagnostic screening purposes.
烟雾病目前缺乏合适的早期临床筛查方法。本研究旨在通过调查外周血外泌体携带的微小RNA来确定一种简单可行的临床筛查指标。实验对象参与静脉采血，使用Exquick相关技术分离外泌体。对提取的外泌体核糖核酸（RNA）进行测序以鉴定差异微小RNA。结果验证涉及从基因数据库中选择相关样本。该研究成功确定了一种早期筛查的潜在标志物，即外周血外泌体携带的hsa-miR-328-3p + hsa-miR-200c-3p。靶基因的富集分析揭示了其与细胞间连接、细胞骨架调节受损和成纤维细胞增殖增加的关联，导致双侧颈内动脉内膜增生和进行性狭窄。这些发现确立了hsa-miR-328-3p+hsa-miR-200c-3p在烟雾病筛查中的诊断价值，同时也有助于更深入地了解其潜在的病理生理学。烟雾病患者与对照受试者之间在外周血外泌体衍生的微小RNA表达上存在显著差异。因此，外周血外泌体，特别是hsa-miR-328-3p + hsa-miR-200c-3p，在诊断筛查方面具有潜在应用价值。
