The location of a mutation affecting ribosomal protein synthesis by Escherichia coli.

A mutation in a strain of Escherichia coli 15 produced ribosomes by an abnormal pathway that caused the accumulation of 47S ribonucleoprotein particles. The mutation was transferred to strains of E. coli K12 by transductions with bacteriophage P1cam and was at about 82 min, between cysE and pyrE and rather closer to the latter. The location and the physiological properties of the mutant suggested that the mutation was in the rpmB,G transcription unit and affected the synthesis of ribosomal proteins L28 and L33.