Lee J S, An G, Friesen J D, Isono K
Mol Gen Genet. 1981;184(2):218-23. doi: 10.1007/BF00272908.
The specialized transducing bacteriophage lambda dpyrE DNA was used as a source of DNA to clone two ribosomal protein genes rpmB (L28) and rpmG (L33) on the cloning vehicle pACYC184. Using one of these plasmids, the nucleotide sequence of these two genes and their flanking regions were determined. The amino acid sequences of both proteins deduced from the nucleotide sequences match with the amino acid sequences previously determined, with one exception. The nucleotide sequences suggest that these two ribosomal protein genes are cotranstribed. There was no expression of the second gene of the operon, rpmG, in the absence of the 5' sequences adjacent to the first gene, rpmB. Observation of the structure of mRNA also strongly supports the idea that rpmB and rpmG are in a single transcription unit whose order is: rpmBp-rpmB-rpmG-rpmGt.
使用专门的转导噬菌体λdpyrE DNA作为DNA来源，在克隆载体pACYC184上克隆了两个核糖体蛋白基因rpmB（L28）和rpmG（L33）。利用其中一个质粒，测定了这两个基因及其侧翼区域的核苷酸序列。从核苷酸序列推导的两种蛋白质的氨基酸序列与先前确定的氨基酸序列相符，但有一个例外。核苷酸序列表明这两个核糖体蛋白基因是共转录的。在没有与第一个基因rpmB相邻的5'序列的情况下，操纵子的第二个基因rpmG没有表达。对mRNA结构的观察也有力地支持了rpmB和rpmG处于单个转录单元的观点，其顺序为：rpmBp-rpmB-rpmG-rpmGt。
