L. is one of the most important medicinal and vegetable crops due to its essential oil, pleasant aroma and taste. In this study, we evaluated the impact of different light intensities, including 100 %, 50 %, and 30 % of natural sunlight, on the growth, phytochemical compositions, and antioxidant activity of green and purple basil cultivars at two different harvest times: early morning and noon. The height of the plant, number of leaves per plant, length of the petiole, diameter of the stem, and fresh and dry weight of the shoot were all reduced by decreasing light intensity in both basil cultivars. When the plants of both cultivars were grown under full light intensity and were sampled at noon, they showed the highest phenolic and flavonoid contents. The highest antioxidant activity was detected in purple basil cultivars grown under 30 and 50 % of sunlight in both harvests. The green basil cultivar showed the highest antioxidant activity when exposed to 30 % sunlight and harvested in the early morning. The highest essential oil content and yield in both basil cultivars were obtained under full sunlight in the early morning harvests. In summary, light intensity and harvest time influence the phytochemical yield, composition, and growth of two studied basil cultivars. Optimal results, particularly for medicinal purposes, were achieved by morning harvesting to maximize the essential oil yield of basils.