Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241002, China.
Chem Commun (Camb). 2024 Jun 27;60(53):6793-6796. doi: 10.1039/d4cc02133a.
We report an integrated ratiometric lysosomal nitric oxide (NO) nanoprobe based on engineered semiconducting polymer dots (Pdots), LyNO-Pdots, which consist of a newly designed NO-responsive dye, a fluorescent conjugated polymer and two functional polymers. The developed probe LyNO-Pdots exhibit high specificity and stability, good photostability and favorable blood-brain barrier (BBB) penetration ability. The LyNO-Pdots are successfully applied to ratiometric imaging of lysosomal NO variations in brain-derived endothelial cells, brain tissues and mice brains with Alzheimer's disease (AD). The results demonstrate that the NO content in the brains of AD mice is considerably higher than that in normal mice.
我们报告了一种基于工程化半导体聚合物点（Pdots）的整合的比率型溶酶体一氧化氮（NO）纳米探针 LyNO-Pdots，它由新设计的对 NO 响应的染料、荧光共轭聚合物和两种功能聚合物组成。所开发的探针 LyNO-Pdots 表现出高特异性和稳定性、良好的光稳定性和有利的血脑屏障（BBB）穿透能力。LyNO-Pdots 成功地应用于脑源性内皮细胞、脑组织和阿尔茨海默病（AD）小鼠脑中溶酶体 NO 变化的比率成像。结果表明，AD 小鼠大脑中的 NO 含量明显高于正常小鼠。
