Student Research Committee, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Hospital Administration Research Center, Sari Branch, Islamic Azad University, Sari, Iran.
Cell Tissue Bank. 2024 Sep;25(3):785-804. doi: 10.1007/s10561-024-10143-2. Epub 2024 Jun 13.
Severe burns often result in an exacerbated inflammatory response, which can contribute to further injury. This inflammatory response may lead to an increased risk of infection, multiple organ failure, and death. This study aimed to investigate the potential of reducing inflammation to enhance burn wound healing in rats using ovine's small intestinal submucosa as a carrier for Wharton's jelly mesenchymal stem cells (WJ-MSCs) and Mineral Pitch (MP). A rat burn model was developed, and the animals were divided into four groups: control group: burn, placebo group: scaffold-treated burn, cell experimental group: WJ-MSCs seeded scaffold-treated burn, and cell and MP experimental group: scaffolds loaded with WJ-MSCs and MP-treated burn. After treating the wounds in the relevant groups and sampling them on days 5, 14 and 21, histological and pathological parameters, and the expression of genes involved in angiogenesis and epithelialization were evaluated. The study results revealed several findings in the burn wounds. These included changes in mast cell populations, a decrease in inflammatory neutrophils and lymphocytes, an increase in fibroblasts and blood vessels, and upregulation of angiogenesis and epithelialization genes. These changes collectively contributed to enhanced wound healing in cell and MP experimental group compared to the other groups. The findings suggest that scaffolds loaded with Wharton's jelly-derived stem cells and MP can serve as engineered tools to modulate inflammatory conditions during the burn wound healing process. These interventions can improve burn wound management and promote better outcomes.
严重烧伤通常会导致炎症反应加剧，从而进一步加重损伤。这种炎症反应可能会增加感染、多器官衰竭和死亡的风险。本研究旨在探讨使用绵羊小肠黏膜作为载体携带 Wharton 氏胶间充质干细胞 (WJ-MSCs) 和 Mineral Pitch (MP) 来减轻炎症反应，从而增强大鼠烧伤创面愈合的潜力。建立大鼠烧伤模型，将动物分为四组：对照组：烧伤；安慰剂组：支架处理烧伤；细胞实验组：WJ-MSCs 接种支架处理烧伤；细胞和 MP 实验组：支架负载 WJ-MSCs 和 MP 处理烧伤。在对相关组的伤口进行处理并在第 5、14 和 21 天取样后，评估了组织学和病理学参数以及参与血管生成和上皮化的基因的表达。研究结果揭示了烧伤伤口中的一些变化。这些变化包括肥大细胞群的变化、炎症中性粒细胞和淋巴细胞的减少、成纤维细胞和血管的增加以及血管生成和上皮化基因的上调。与其他组相比，细胞和 MP 实验组的这些变化共同促进了伤口愈合的增强。研究结果表明，负载 Wharton 氏胶衍生干细胞和 MP 的支架可以作为工程工具，调节烧伤伤口愈合过程中的炎症状态。这些干预措施可以改善烧伤伤口的管理，促进更好的结果。
