人脐带华通氏胶间充质干细胞通过旁分泌信号促进皮肤伤口愈合。

Human Wharton's jelly mesenchymal stem cells promote skin wound healing through paracrine signaling.

作者信息

Arno Anna I, Amini-Nik Saeid, Blit Patrick H, Al-Shehab Mohammed, Belo Cassandra, Herer Elaine, Tien Col Homer, Jeschke Marc G

出版信息

Stem Cell Res Ther. 2014 Feb 24;5(1):28. doi: 10.1186/scrt417.

INTRODUCTION

The prevalence of nonhealing wounds is predicted to increase due to the growing aging population. Despite the use of novel skin substitutes and wound dressings, poorly vascularized wound niches impair wound repair. Mesenchymal stem cells (MSCs) have been reported to provide paracrine signals to promote wound healing, but the effect of human Wharton's jelly-derived MSCs (WJ-MSCs) has not yet been described in human normal skin.

METHODS

Human WJ-MSCs and normal skin fibroblasts were isolated from donated umbilical cords and normal adult human skin. Fibroblasts were treated with WJ-MSC-conditioned medium (WJ-MSC-CM) or nonconditioned medium.

RESULTS

Expression of genes involved in re-epithelialization (transforming growth factor-β2), neovascularization (hypoxia-inducible factor-1α) and fibroproliferation (plasminogen activator inhibitor-1) was upregulated in WJ-MSC-CM-treated fibroblasts (P≤0.05). WJ-MSC-CM enhanced normal skin fibroblast proliferation (P≤0.001) and migration (P≤0.05), and promoted wound healing in an excisional full-thickness skin murine model.

CONCLUSIONS

Under our experimental conditions, WJ-MSCs enhanced skin wound healing in an in vivo mouse model.

引言

由于老龄化人口不断增加，预计难愈性伤口的患病率将会上升。尽管使用了新型皮肤替代物和伤口敷料，但血管化不良的伤口微环境会损害伤口修复。据报道，间充质干细胞（MSC）可提供旁分泌信号促进伤口愈合，但人脐带华通氏胶来源的间充质干细胞（WJ-MSC）在人正常皮肤中的作用尚未见报道。

方法

从捐赠的脐带和正常成人皮肤中分离出人WJ-MSC和正常皮肤成纤维细胞。用WJ-MSC条件培养基（WJ-MSC-CM）或非条件培养基处理成纤维细胞。

结果

在WJ-MSC-CM处理的成纤维细胞中，参与再上皮化（转化生长因子-β2）、新血管形成（缺氧诱导因子-1α）和纤维增殖（纤溶酶原激活物抑制剂-1）的基因表达上调（P≤0.05）。WJ-MSC-CM增强了正常皮肤成纤维细胞的增殖（P≤0.001）和迁移（P≤0.05），并在切除性全层皮肤小鼠模型中促进了伤口愈合。