Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman.
Methods Mol Biol. 2024;2832:241-256. doi: 10.1007/978-1-0716-3973-3_18.
Identification and isolation of plant growth-promoting bacteria (PGPB) are critical steps toward understanding the role of these bacteria in stress tolerance in plants. This procedure also provides essential knowledge about the microbes needed to formulate effective biofertilizers. This chapter describes culture-dependent and culture-independent strategies to identify and isolate PGPB. The culture-dependent strategy commonly involves growing PGPB on general and selective media. However, the culture-independent strategy involves next-generation sequencing technologies. A combination of both strategies would identify the structure of the bacterial communities and isolate bacteria from their environments. Therefore, this chapter describes a comprehensive strategy where the methods are sequentially applied to identify and isolate epiphytic and endophytic PGPB from a particular environmental sample. However, a single procedure can also be employed to identify and isolate a specific type of PGPB.
鉴定和分离植物促生菌（PGPB）是理解这些细菌在植物胁迫耐受中作用的关键步骤。该过程还为制定有效生物肥料所需的微生物提供了必要的知识。本章描述了鉴定和分离 PGPB 的依赖培养和非依赖培养策略。依赖培养策略通常涉及在一般和选择性培养基上培养 PGPB。然而，非依赖培养策略涉及下一代测序技术。两种策略的结合将确定细菌群落的结构，并从其环境中分离细菌。因此，本章描述了一种综合策略，其中方法依次应用于从特定环境样本中鉴定和分离附生和内生 PGPB。然而，也可以采用单一程序来鉴定和分离特定类型的 PGPB。
