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基于偶联的辣根过氧化物酶和二胺氧化酶仿生级联酶的杂化微球用于二胺的比色检测。

Colorimetric detection of diamine using diamine oxidase and horseradish peroxidase co-incorporated hybrid microsphere as biomimetic cascade enzymes.

机构信息

Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.

Jiangsu Topole Biotechnology Co., Ltd, Xuzhou 221000, China.

出版信息

Food Chem. 2024 Oct 30;456:140008. doi: 10.1016/j.foodchem.2024.140008. Epub 2024 Jun 7.

Abstract

Dual-enzyme co-embedded materials have shown high potential for achieving efficient detection due to the convenience of two-enzyme cascade reactions. Herein, we developed a dual-enzyme hybrid microsphere (HM) based biosensor to detect diamines (histamine was included for ease of description) in aquatic products. The HM was made from diamine oxidase, horseradish peroxidase, and copper phosphate through the biomineralization method. Under optimal conditions, the system displayed linear color response to histamine of different concentrations ranging from 0 to 200 μg/mL. The detection limit of histamine was 0.15 μg/mL, showing higher sensitivity than the two-step free enzyme assay. Moreover, the detection system exhibited good specificity to diamines. The method was used to detect diamines in commercial samples, and the results were compared with those measured by the high-performance liquid chromatography method. Overall, the proposed assay exhibited high potential in diamine quantification and was readily extended to other cascade enzymatic reaction-based detection strategies.

摘要

双酶共嵌入材料由于双酶级联反应的便利性,在实现高效检测方面显示出巨大的潜力。本文构建了一种基于双酶混合微球(HM)的生物传感器,用于检测水产品中的二胺(以组氨酸为例进行说明)。HM 通过生物矿化法由二胺氧化酶、辣根过氧化物酶和磷酸铜制成。在最佳条件下,该系统对不同浓度(0 至 200μg/mL)的组氨酸表现出线性颜色响应。组氨酸的检测限为 0.15μg/mL,比两步游离酶法更灵敏。此外,该检测系统对二胺具有良好的特异性。该方法用于检测商业样品中的二胺,并将结果与高效液相色谱法的测量结果进行比较。总的来说,该测定方法在二胺定量方面具有很大的潜力,并且可以很容易地扩展到其他基于级联酶反应的检测策略。

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