Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Nanoscale. 2014 Jan 7;6(1):255-62. doi: 10.1039/c3nr04425d. Epub 2013 Nov 1.
This study reports a facile method for the synthesis of multi-enzyme co-embedded organic-inorganic hybrid nanoflowers, using glucose oxidase (GOx) and horseradish peroxidase (HRP) as the organic components, and Cu3(PO4)2 · 3H2O as the inorganic component. The synthesized nanoflowers enable the combination of a two-enzyme cascade reaction in one step, in which the GOx component of the nanoflowers oxidizes glucose to generate H2O2, which then reacts with the adjacent HRP component on the nanoflowers to oxidize the chromogenic substrates, resulting in an apparent color change. Given the close proximity of the two enzyme components in a single nanoflower, this novel sensor greatly reduces the diffusion and decomposition of H2O2, and greatly enhances the sensitivity of glucose detection. Thus, the obtained multi-enzyme co-embedded organic-inorganic hybrid nanoflowers can be unquestionably used as highly sensitive colorimetric sensors for the detection of glucose. Notably, this work presents a very facile route for the synthesis of multi-enzyme co-embedded nanomaterials for the simultaneous catalysis of multi-step cascade enzymatic reactions. Furthermore, it has great potential for application in biotechnology, and biomedical and environmental chemistry.
本研究报告了一种简便的方法，用于合成多酶共嵌入的有机-无机杂化纳米花，使用葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP）作为有机成分，和 Cu3(PO4)2·3H2O 作为无机成分。所合成的纳米花使两步级联反应能够在一步中结合，其中纳米花的 GOx 成分将葡萄糖氧化生成 H2O2，然后与纳米花上相邻的 HRP 成分反应，氧化显色底物，导致明显的颜色变化。鉴于单个纳米花中两种酶成分的紧密接近，这种新型传感器大大减少了 H2O2 的扩散和分解，并大大提高了葡萄糖检测的灵敏度。因此，所获得的多酶共嵌入的有机-无机杂化纳米花可以毫无疑问地用作检测葡萄糖的高灵敏度比色传感器。值得注意的是，这项工作为同时催化多步级联酶反应的多酶共嵌入纳米材料的合成提供了一种非常简便的途径。此外，它在生物技术、生物医学和环境化学领域具有很大的应用潜力。
