Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, The School of Pharmacy, Fujian Medical University, Fuzhou, 350108, China.
State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Biosens Bioelectron. 2024 Oct 1;261:116492. doi: 10.1016/j.bios.2024.116492. Epub 2024 Jun 10.
Exosomes have been considered as promising biomarkers for cancer diagnosis due to their abundant information from originating cells. However, sensitive and reliable detection of exosomes is still facing technically challenges due to the lack of a sensing platform with high sensitivity and reproducibility. To address the challenges, here we propose a portable surface plasmon resonance (SPR) sensing of exosomes with a three-layer Au mirror/SiO spacer/Au nanohole sensor, fabricated by an economical polystyrene nanosphere self-assembly method. The SiO spacer can act as an optical cavity and induce mode hybridization, leading to excellent optimization of both sensitivity and full width at half maximum compared with normal single layer Au nanohole sensors. When modified with CD63 or EpCAM aptamers, a detection of limit (LOD) of as low as 600 particles/μL was achieved. The sensors showed good capability to distinguish between non-tumor derived L02 exosomes and tumor derived HepG2 exosomes. Additionally, high reproducibility was also achieved in detection of artificial serum samples with RSD as low as 2%, making it feasible for clinical applications. This mode hybridization plasmonic sensor provides an effective approach to optimize the detection sensitivity of exosomes, pushing SPR sensing one step further towards cancer diagnosis.
外泌体因其具有源自细胞的丰富信息，被认为是很有前途的癌症诊断生物标志物。然而，由于缺乏具有高灵敏度和重现性的传感平台，对外泌体的敏感和可靠检测仍然面临技术挑战。为了解决这些挑战，我们提出了一种基于三层金镜/SiO 间隔层/Au 纳米孔传感器的便携式表面等离子体共振（SPR）外泌体传感方法，该方法通过经济的聚苯乙烯纳米球自组装方法制备。SiO 间隔层可以作为光学腔并诱导模式杂化，与普通单层 Au 纳米孔传感器相比，灵敏度和半峰全宽得到了极好的优化。当用 CD63 或 EpCAM 适体修饰后，检测限（LOD）低至 600 个/μL。该传感器具有良好的区分非肿瘤源性 L02 外泌体和肿瘤源性 HepG2 外泌体的能力。此外，在检测人工血清样本时，还具有较高的重现性，RSD 低至 2%，使其具有临床应用的可行性。这种模式杂化等离子体传感器为优化外泌体的检测灵敏度提供了一种有效的方法，推动 SPR 传感在癌症诊断方面更进一步。
