Sousa J, Nath J, Tucker J D, Ong T M
Mutat Res. 1985 May;149(3):365-74. doi: 10.1016/0027-5107(85)90153-8.
Studies have been conducted to determine whether the mutagens in fried beef ingested by human subjects are excreted in the urine. Urine samples were collected from individuals on liquid or regular diets before and after a fried beef meal. The mutagenic activity of the samples was tested in the Ames Salmonella/microsome assay system. The results showed that in individuals on liquid diets, most of the urinary mutagenic activity is recovered within 2-6 h after consuming a fried beef meal. In one individual tested, mutagenic activity was found in urine samples obtained 6-15 h after the fried beef meal. No mutagenic activity was detected in any of the urine samples obtained 15-24 h following the meal. In individuals on a regular diet, however, mutagenic activity was frequently observed in urine samples obtained 16-24 h following the fried beef meal, although the mutagenic activity was not as great as that in the preceding 16 h. It appears that the mutagenic agents generated by the frying of beef are ingested, absorbed, and excreted by the human body in biologically detectable quantities. These results suggest that subjects should abstain from fried beef at least one day prior to and during urine mutagenicity screening.
已经开展了多项研究来确定人类受试者摄入的油炸牛肉中的诱变剂是否会通过尿液排出。在食用油炸牛肉餐前后，从食用流食或常规饮食的个体中收集尿液样本。在艾姆斯沙门氏菌/微粒体检测系统中测试样本的诱变活性。结果显示，对于食用流食的个体，大部分尿液诱变活性在食用油炸牛肉餐后2至6小时内恢复。在一名受试个体中，在油炸牛肉餐后6至15小时采集的尿液样本中发现了诱变活性。餐后15至24小时采集的任何尿液样本中均未检测到诱变活性。然而，对于食用常规饮食的个体，在油炸牛肉餐后16至24小时采集的尿液样本中经常观察到诱变活性，尽管诱变活性不如前16小时那么高。看来，油炸牛肉产生的诱变剂会被人体摄入、吸收并以生物可检测的量排出。这些结果表明，在进行尿液诱变筛查之前至少一天以及筛查期间，受试者应避免食用油炸牛肉。
