Björklund H, Bignami A, Dahl D
Neurosci Lett. 1985 Mar 15;54(2-3):363-8.
The presence of glial fibrillary acidic protein (GFA)-positive Müller glia and retinal astrocytes were studied immunohistochemically in normal rat retina. Using GFA antiserum both Müller glia and separate star-shaped cells were observed in spread-preparations as well as cryostat sections. The retinal astrocytes were also visualized using two different monoclonal GFA antibodies. These cells were found to be located in the nerve fiber and ganglion cell layers. In contrast, Müller glia were not normally visualized with any of the monoclonal GFA antibodies but could be stained 4 days after an optic nerve crush. Our results demonstrate that normal rat Müller glia expresses GFA-like immunoreactivity.
采用免疫组织化学方法研究了正常大鼠视网膜中胶质纤维酸性蛋白（GFA）阳性的 Müller 胶质细胞和视网膜星形胶质细胞。使用 GFA 抗血清，在铺片标本以及低温恒温器切片中均观察到了 Müller 胶质细胞和分散的星形细胞。还使用两种不同的单克隆 GFA 抗体对视网膜星形胶质细胞进行了可视化观察。发现这些细胞位于神经纤维层和神经节细胞层。相比之下，正常情况下任何一种单克隆 GFA 抗体都无法使 Müller 胶质细胞可视化，但在视神经挤压 4 天后可以对其进行染色。我们的结果表明，正常大鼠的 Müller 胶质细胞表达 GFA 样免疫反应性。
