Dahl D, Bignami A
J Histochem Cytochem. 1982 Mar;30(3):207-13. doi: 10.1177/30.3.7037941.
The distribution of glial fibrillary acidic (GFA) protein and desmin was compared in cryostat sections of rat brain, spinal cord, and eye by immunofluorescence and peroxidase-antiperoxidase (PAP) staining. Desmin antisera were raised to antigen purified from chicken gizzard. In rat brain and spinal cord, GFA protein and desmin were selectively localized in astrocytes. Neurons and axons were not stained. The only difference between GFA and desmin antisera was the staining of smooth muscle in small arteries with anti-desmin. It was only in retinal glia that a difference in the localization of the two proteins was apparent. As previously reported, only the glia limitans on the inner surface of the retina was demonstrated with GFA antisera in the normal eye. With anti-desmin Müller fibers spanning the whole thickness of the retina were stained. It is concluded that GFA and desmin form two distinct systems of 100 A filaments in astroglia, as previously reported for GFA and vimentin.
通过免疫荧光和过氧化物酶-抗过氧化物酶（PAP）染色，比较了大鼠脑、脊髓和眼的低温切片中胶质纤维酸性（GFA）蛋白和结蛋白的分布。针对从鸡胗中纯化的抗原制备了结蛋白抗血清。在大鼠脑和脊髓中，GFA蛋白和结蛋白选择性地定位于星形胶质细胞。神经元和轴突未被染色。GFA和结蛋白抗血清之间的唯一区别是抗结蛋白对小动脉平滑肌的染色。仅在视网膜神经胶质中，两种蛋白的定位差异才明显。如先前报道，在正常眼中，GFA抗血清仅显示视网膜内表面的神经胶质界膜。用抗结蛋白染色时，横跨视网膜全层的米勒纤维被染色。结论是，如先前关于GFA和波形蛋白的报道，GFA和结蛋白在星形胶质细胞中形成两个不同的100 A细丝系统。
