Shen Feng, Ying Jiali, Xu Liang, Sun Xiaochuan, Wang Jizhong, Wang Yan, Mei Yi, Zhu Yuelin, Liu Liwang
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China) of MOAR, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095 China.
Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng, 224002 China.
Physiol Mol Biol Plants. 2021 Sep;27(9):2027-2041. doi: 10.1007/s12298-021-01056-5. Epub 2021 Sep 14.
Plant annexins are a kind of conserved Ca-dependent phospholipid-binding proteins which are involved in plant growth, development and stress tolerance. Radish is an economically important annual or biennial root vegetable crop worldwide. However, the genome-wide characterization of () gene family remain largely unexplored in radish. In this study, a comprehensive identification of a gene family was performed at the whole genome level in radish. In total, ten genes were identified, and these putative RsANN proteins shared typical characteristics of the annexin family proteins. Phylogenetic analysis showed that the RsANNs together with annexin from Arabidopsis and rice were clustered into five groups with shared similar motif patterns. Chromosomal localization showed that these ten genes were distributed on six chromosomes (R3-R8) of radish. Several -elements involved in abiotic stress response were identified in the promoter regions of genes. Expression profile analysis indicated that the genes exhibited tissue-specific patterns at different growth stages and tissues. The Real-time quantitative PCR (RT-qPCR) revealed that the expression of most genes was induced under various abiotic stresses including heat, drought, salinity, oxidization and ABA stress. In addition, stress assays showed that overexpression of improved plant's growth and heat tolerance, while artificial microRNAs (amiRNA)-mediated knockdown of caused dramatically decreased survival ratio of Arabidopsis plants. These findings not only demonstrate that might play a critical role in the heat stress response of radish, but also facilitate clarifying the molecular mechanism of genes in regulating the biological process governing plant growth and development.
The online version contains supplementary material available at 10.1007/s12298-021-01056-5.
植物膜联蛋白是一类保守的钙依赖性磷脂结合蛋白，参与植物的生长、发育和抗逆性。萝卜是全球一种重要的经济作物，属于一年生或二年生根菜类蔬菜。然而，萝卜中（）基因家族的全基因组特征在很大程度上仍未被探索。在本研究中，对萝卜全基因组水平的一个基因家族进行了全面鉴定。总共鉴定出10个基因，这些推测的RsANN蛋白具有膜联蛋白家族蛋白的典型特征。系统发育分析表明，RsANNs与拟南芥和水稻的膜联蛋白一起被聚类为五组，具有相似的基序模式。染色体定位表明，这10个基因分布在萝卜的6条染色体（R3 - R8）上。在这些基因的启动子区域鉴定出了几个参与非生物胁迫响应的元件。表达谱分析表明，这些基因在不同生长阶段和组织中呈现出组织特异性模式。实时定量PCR（RT - qPCR）显示，大多数基因的表达在包括热、干旱、盐度、氧化和脱落酸胁迫在内的各种非生物胁迫下被诱导。此外，胁迫试验表明，该基因的过表达提高了植物的生长和耐热性，而人工微小RNA（amiRNA）介导的该基因敲低导致拟南芥植株的存活率显著降低。这些发现不仅表明该基因可能在萝卜的热胁迫响应中起关键作用，而且有助于阐明该基因在调节植物生长发育生物学过程中的分子机制。
在线版本包含可在10.1007/s12298 - 021 - 01056 - 5获取的补充材料。
