Eskandani Morteza, Jahanban-Esfahlan Rana, Sadughi Mohammad Mehdi, Jaymand Mehdi
Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Medical Biotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Heliyon. 2024 May 30;10(11):e32183. doi: 10.1016/j.heliyon.2024.e32183. eCollection 2024 Jun 15.
A novel thermal-responsive β-cyclodextrin-based magnetic hydrogel [β-cyclodextrin--poly(-isopropylacrylamide)/FeO (β-CD--PNIPAAm/FeO)] was fabricated as a novel nanomedicine for chemo/hyperthermia treatment of cancer cells. Firstly, β-CD was modified by maleic anhydride (MA) followed by copolymerization with NIPAAm monomer and thiol-end capped FeO nanoparticles (NPs) in the presence of a crosslinker through acrylamide-thiol polymerization system to afford a magnetic hydrogel. The saturation magnetization ( ) value for developed hydrogel was determined to be 8.2 emu g. The hydrogel was physically loaded with an anticancer agent, doxorubicin hydrochloride (Dox). The encapsulation efficiency (EE) of drug into the hydrogel was obtained as 73 %. The system represented acceptable thermal-triggered drug release behavior that best fitted with Higuchi model, demonstrating the release of drug is mostly controlled by diffusion mechanism. The anticancer performance of the β-CD--PNIPAAm/FeO-Dox was evaluated using MCF7 cells by MTT-assay. In addition, flow cytometry analyses showed considerable cellular uptake of Dox in the cells treated with β-CD--PNIPAAm/FeO-Dox (∼70 %) compared to free Dox (∼28 %). As results, in time period of 48 h by combination of chemo- and hyperthermia-therapies, the developed system displayed greater anticancer efficiency than the free Dox.
一种新型的基于热响应性β-环糊精的磁性水凝胶[β-环糊精-聚（N-异丙基丙烯酰胺）/Fe₃O₄（β-CD--PNIPAAm/Fe₃O₄）]被制备出来，作为一种用于癌细胞化学/热疗的新型纳米药物。首先，β-环糊精用马来酸酐（MA）进行改性，然后在交联剂存在下，通过丙烯酰胺-硫醇聚合体系与NIPAAm单体和硫醇封端的Fe₃O₄纳米粒子（NPs）共聚，得到磁性水凝胶。所制备水凝胶的饱和磁化强度（ ）值测定为8.2 emu g。该水凝胶物理负载了抗癌剂盐酸多柔比星（Dox）。药物包封入水凝胶的包封率（EE）为73%。该体系表现出可接受的热触发药物释放行为，最符合Higuchi模型，表明药物释放主要受扩散机制控制。通过MTT法使用MCF7细胞评估了β-CD--PNIPAAm/Fe₃O₄-Dox的抗癌性能。此外，流式细胞术分析表明，与游离Dox（约28%）相比，用β-CD--PNIPAAm/Fe₃O₄-Dox处理的细胞对Dox的细胞摄取量相当可观（约70%）。结果表明，在48小时的时间内，通过化学疗法和热疗相结合，所开发的体系显示出比游离Dox更高的抗癌效率。
