Shapira Z, Shohat B, Yussim A, Shmueli D, Servadio C
Proc Eur Dial Transplant Assoc Eur Ren Assoc. 1985;21:1037-41.
Peripheral T cell subsets were determined in 26 patients after renal transplantation. Thirteen patients were undergoing acute rejection episodes at the time of blood sampling. Two methods were used for the determinations: the theophylline sensitivity test (THST) and the monoclonal antibodies method OKT8 and OKT4. In both rejecting and non-rejecting groups, the percentages of T suppressor lymphocytes (TS) was found to be higher by the OKT8 method than by the THST. Furthermore, no significant difference in TS percentages could be revealed by the OKT8 method between the two groups. However, with the THST the differences were significant with a mean value of 13.5 +/- 7.9 per cent in the rejecting group and 21 +/- 5.9 per cent in the non-rejecting group (p less than 0.01). The different results between the two methods could be attributed to the fact that by THST only TS cells are defined while the OKT8 conjugates also with cytotoxic T lymphocytes. The measuring of T helper cells (TH) revealed much higher percentages of TH in the rejecting group than in the non-rejecting group, 51 +/- 9.5 per cent mean value and 29 +/- 13 per cent mean value, respectively (p less than 0.05). The ratio OKT4+/OKT8+ was below one in the non-rejecting group and above 1.5 in the rejecting group. We concluded that the THST as well as OKT4+/OKT8+ ratio may be a helpful laboratory test to confirm a clinically suspected acute rejection episode.
对26例肾移植患者的外周血T细胞亚群进行了检测。13例患者在采血时正处于急性排斥反应期。采用两种方法进行检测：茶碱敏感性试验（THST）和单克隆抗体法OKT8及OKT4。在排斥组和非排斥组中，OKT8法检测出的T抑制淋巴细胞（TS）百分比均高于THST法。此外，OKT8法检测两组间TS百分比无显著差异。然而，THST法检测两组间差异显著，排斥组平均值为13.5±7.9%，非排斥组为21±5.9%（p<0.01）。两种方法结果不同的原因可能是，THST仅能界定TS细胞，而OKT8还能与细胞毒性T淋巴细胞结合。T辅助细胞（TH）的检测显示，排斥组TH百分比远高于非排斥组，平均值分别为51±9.5%和29±13%（p<0.05）。非排斥组OKT4+/OKT8+比值低于1，排斥组高于1.5。我们得出结论，THST以及OKT4+/OKT8+比值可能是有助于确诊临床疑似急性排斥反应的实验室检测指标。
