Ailhaud G, Amri E, Czerucka D, Forest C, Gaillard D, Grimaldi P, Négrel R, Vannier C
Reprod Nutr Dev (1980). 1985;25(1B):153-8.
Some hormonal factors, possibly involved in the proliferation and differentiation of adipose precursor cells in vivo, have been characterized in vitro using different preadipocyte cell lines established from rodent adipose tissue. The process of adipose conversion has also been studied using these cell lines; in this process, stem cells (adipoblasts) were committed at any cell division during the growth phase. At confluence, committed cells (preadipocytes) underwent a limited number of mitoses and differentiated into adipose cells, whereas the uncommitted cells remained as stem cells in the cell population. This stochastic model could be extended to the development of rat adipose tissue in vivo. The study of adipose conversion showed the early emergence of lipoprotein lipase (LPL) and monoglyceride lipase (MGL). LPL activity appeared in the cells before any triglyceride accumulation. In contrast, this accumulation seemed dependent upon the emergence of glycerol-3-phosphate dehydrogenase. In vitro experiments clearly established that LPL-containing (differentiating) cells underwent postconfluent mitoses. This limited proliferation was in agreement with previous data obtained in vivo and indicates that only triglyceride-containing (mature) cells could not divide.
一些可能参与体内脂肪前体细胞增殖和分化的激素因子，已通过使用从啮齿动物脂肪组织建立的不同前脂肪细胞系在体外进行了表征。脂肪转化过程也已使用这些细胞系进行了研究；在这个过程中，干细胞（成脂肪细胞）在生长阶段的任何细胞分裂时都会定向分化。汇合时，定向细胞（前脂肪细胞）经历有限次数的有丝分裂并分化为脂肪细胞，而未定向的细胞则作为干细胞保留在细胞群体中。这种随机模型可以扩展到体内大鼠脂肪组织的发育。脂肪转化研究表明脂蛋白脂肪酶（LPL）和甘油单酯脂肪酶（MGL）的早期出现。在任何甘油三酯积累之前，LPL活性就出现在细胞中。相比之下，这种积累似乎依赖于3-磷酸甘油脱氢酶的出现。体外实验清楚地表明，含有LPL的（正在分化的）细胞在汇合后会进行有丝分裂。这种有限的增殖与先前在体内获得的数据一致，表明只有含有甘油三酯的（成熟）细胞不能分裂。
