Opt Lett. 2024 Jun 15;49(12):3356-3359. doi: 10.1364/OL.523570.
Mueller matrix microscopy can provide comprehensive polarization-related optical and structural information of biomedical samples label-freely. Thus, it is regarded as an emerging powerful tool for pathological diagnosis. However, the staining dyes have different optical properties and staining mechanisms, which can put influence on Mueller matrix microscopic measurement. In this Letter, we quantitatively analyze the polarization enhancement mechanism from hematoxylin and eosin (H&E) staining in multispectral Mueller matrix microscopy. We examine the influence of hematoxylin and eosin dyes on Mueller matrix-derived polarization characteristics of fibrous tissue structures. Combined with Monte Carlo simulations, we explain how the dyes enhance diattenuation and linear retardance as the illumination wavelength changed. In addition, it is demonstrated that by choosing an appropriate incident wavelength, more visual Mueller matrix polarimetric information can be observed of the H&E stained tissue sample. The findings can lay the foundation for the future Mueller matrix-assisted digital pathology.
穆勒矩阵显微镜可以无标记地提供生物医学样本的全面偏振相关的光学和结构信息。因此，它被认为是一种用于病理诊断的新兴强大工具。然而，染色染料具有不同的光学性质和染色机制，这可能会对穆勒矩阵显微镜测量产生影响。在这封信件中，我们定量分析了多光谱穆勒矩阵显微镜中苏木精和伊红（H&E）染色的偏振增强机制。我们研究了苏木精和伊红染料对纤维组织结构的穆勒矩阵衍生偏振特性的影响。结合蒙特卡罗模拟，我们解释了染料如何随着照明波长的变化而增强双折射和线性延迟。此外，还证明了通过选择适当的入射波长，可以观察到更多的苏木精和伊红染色组织样本的穆勒矩阵偏振信息。这些发现为未来的穆勒矩阵辅助数字病理学奠定了基础。
