Opt Lett. 2024 Jun 15;49(12):3368-3371. doi: 10.1364/OL.515783.
We present a versatile extended depth-of-field (EDOF) wide-field fluorescence microscopy using a new, to the best of our knowledge, active device, micro-mirror array lens system (MALS) for calibration-free and orientation-insensitive EDOF imaging. The MALS changed the focal plane during image acquisition, and the system could be operated in any orientation. Two EDOF imaging modes of high-speed accumulation and low-speed surface sectioning were implemented. The performance was demonstrated in non-contact imaging of conjunctival goblet cells in live mice and depth-resolved cellular examination of ex-vivo human cancer specimens. MALS-based EDOF microscopy has potential for versatile cellular examination.
我们提出了一种通用的扩展景深（EDOF）宽场荧光显微镜，使用了一种新的、据我们所知的主动器件，微镜阵列透镜系统（MALS），用于无校准和无方向敏感性的 EDOF 成像。MALS 在图像采集过程中改变了焦平面，并且系统可以在任何方向上操作。实现了两种 EDOF 成像模式，高速累积和低速表面切片。在活体小鼠的结膜杯状细胞的非接触成像和离体人癌症标本的深度分辨细胞检查中展示了性能。基于 MALS 的 EDOF 显微镜具有用于多功能细胞检查的潜力。
