IEEE Trans Med Imaging. 2022 Aug;41(8):2004-2008. doi: 10.1109/TMI.2022.3151944. Epub 2022 Aug 1.
Conjunctival goblet cells (CGCs) are mucin-secreting cells in the eye and play essential roles for ocular surface homeostasis. Since various ocular surface pathologies are related to CGC dysfunction, CGC examination is important for the evaluation of ocular surface conditions. Recently we introduced moxifloxacin-based fluorescence microscopy (MBFM) for non-invasive CGC imaging. However, the imaging speed was up to 1 frame per second (fps) and needed to be improved for clinical applications. In this study, we developed a high-speed moxifloxacin-based, extended depth-of-field (EDOF) microscopy system that operates at a maximum imaging speed of 15 fps. The system used a deformable mirror for the high-speed axial sweeping of focal plane during single-frame acquisitions. The acquired images contained both in-focus and out-of-focus information, and deconvolution was used to filter the in-focus information. The system had a DOF of 800 [Formula: see text], field-of-view of 1.2 mm ×1.2 mm, and resolution of [Formula: see text]. Its performance was demonstrated by real-time, breathing-motion-insensitive CGC imaging of mouse and rabbit models, in vivo. High-speed EDOF microscopy has potentials for non-invasive, real-time CGC examinations of human subjects.
结膜杯状细胞（CGC）是眼睛中分泌黏液的细胞，对于眼表面的稳态起着至关重要的作用。由于各种眼表面疾病都与 CGC 功能障碍有关，因此对 CGC 的检查对于评估眼表面状况非常重要。最近，我们引入了基于莫西沙星的荧光显微镜（MBFM）用于非侵入性的 CGC 成像。然而，其成像速度高达每秒 1 帧（fps），需要进行改进以应用于临床。在这项研究中，我们开发了一种高速、基于莫西沙星的扩展景深（EDOF）显微镜系统，其最大成像速度可达 15 fps。该系统在单次采集过程中使用变形镜对焦平面进行高速轴向扫描。所获取的图像包含聚焦和离焦信息，通过反卷积来过滤聚焦信息。该系统的景深为 800 [Formula: see text]，视野为 1.2 mm×1.2 mm，分辨率为 [Formula: see text]。通过对小鼠和兔模型进行实时、呼吸运动不敏感的 CGC 成像，验证了该系统的性能。高速 EDOF 显微镜具有对人类受试者进行非侵入性、实时 CGC 检查的潜力。
