新型隐球菌原生质体的制备与再生
Production and regeneration of protoplasts from Cryptococcus.
作者信息
Rhodes J C, Kwon-Chung K J
出版信息
Sabouraudia. 1985 Feb;23(1):77-80.
PMID:3887604
Abstract
Protoplasts were quickly and efficiently produced from both varieties of Cryptococcus neoformans and from C. laurentii by use of the multi-enzyme product Novozym 234. Conditions for regeneration of protoplasts are described. DNA yield from the Novozym-produced protoplasts was superior to that from snail gut enzyme-derived protoplasts.
摘要
通过使用多酶产品诺维信234，从新型隐球菌的两个变种以及罗伦隐球菌中快速且高效地制备出了原生质体。文中描述了原生质体的再生条件。诺维信制备的原生质体的DNA产量优于蜗牛肠道酶制备的原生质体。
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