须癣毛癣菌菌丝体和小分生孢子原生质体的超微结构

Ultrastructure of protoplasts from mycelium and microconidia of Trichophyton mentagrophytes.

作者信息

Scott E M, Gorman S P, Wright L R

出版信息

Sabouraudia. 1985 Feb;23(1):31-6. doi: 10.1080/00362178585380061.

Abstract

Protoplast formation from mycelium and microconidia of Trichophyton mentagrophytes was achieved with Novozym 234. Pretreatment procedures with dithiothreitol or urea mercaptoethanol sodium lauryl sulphate before digestion with Novozym 234 greatly reduced protoplast yield from mycelium. Snail gut enzyme did not protoplasts in good yield. Scanning electron microscopy of mycelium protoplasts showed the acquired spherical shape. The plasma membrane appeared finely granular although remnants of cell wall could sometimes be observed. Transmission electron microscopy showed the cell interior of these protoplasts was plasmolysed. Microconidia treated with Novozym 234 displayed a range of cell wall digestion, with intact protoplasts showing distinct cytoplasmic organelles.

摘要

使用诺维信234从须癣毛癣菌的菌丝体和微分生孢子中成功获得了原生质体。在用诺维信234消化之前，用二硫苏糖醇或尿素巯基乙醇十二烷基硫酸钠进行预处理程序，大大降低了菌丝体的原生质体产量。蜗牛肠道酶不能高效产生原生质体。对菌丝体原生质体进行扫描电子显微镜观察显示其呈获得的球形。尽管有时可以观察到细胞壁残余物，但质膜看起来呈细颗粒状。透射电子显微镜显示这些原生质体的细胞内部发生了质壁分离。用诺维信234处理的微分生孢子表现出一系列细胞壁消化情况，完整的原生质体显示出明显的细胞质细胞器。