Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Int J Biol Macromol. 2024 Jul;273(Pt 2):132972. doi: 10.1016/j.ijbiomac.2024.132972. Epub 2024 Jun 12.
The use of essential oils as natural antioxidant, antimicrobial and insect repellent agent was limited by the loss of bioactive components especially volatile compounds. This study aimed to improve biological properties of curry leaf essential oil (CLEO) by producing nanometer sized particles through two different synthesis techniques; nanoencapsulation and nanoprecipitation. The methods produced different nanostructures; nanocapsules and nanospheres distinguished by the morphological structure (TEM analysis). Successful loading of CLEO into chitosan nanocarrier was proven by FTIR spectra. Zeta potential values for both nanostructures were more than +30 mV implying their stability against aggregation. CLEO loaded nanocapsules exhibited highest antibacterial properties against Gram-positive bacteria compared to nanospheres. Meanwhile, CLEO loaded nanospheres recorded up until 90.44 % DPPH radical scavenging properties, higher compared to nanocapsules. Both nanostructures demonstrated further improvement in antioxidant and antibacterial activities with the incorporation of higher chitosan concentration. In vitro release analysis indicated that CLEO undergo two-stage discharge mechanism where fast discharge occurred up until 12 h followed by sustained released afterwards. The two synthesis methods applied synergistically with greater chitosan concentration successfully produced nanostructures with >60 % encapsulation efficiency (EE). This concluded that both techniques were reliable to protect the bioactive constituents of CLEO for further used.
将精油用作天然抗氧化剂、抗菌剂和驱虫剂的应用受到生物活性成分（尤其是挥发性化合物）损失的限制。本研究旨在通过两种不同的合成技术——纳米胶囊化和纳米沉淀法来提高咖喱叶精油（CLEO）的生物学特性。这两种方法产生了不同的纳米结构；纳米胶囊和纳米球，通过形态结构（TEM 分析）区分。FTIR 光谱证明了成功地将 CLEO 加载到壳聚糖纳米载体中。两种纳米结构的 Zeta 电位值均高于+30 mV，表明它们具有抗聚集的稳定性。与纳米球相比，负载 CLEO 的纳米胶囊对革兰氏阳性菌表现出最高的抗菌性能。同时，负载 CLEO 的纳米球记录到高达 90.44%的 DPPH 自由基清除活性，高于纳米胶囊。随着壳聚糖浓度的增加，两种纳米结构的抗氧化和抗菌活性均得到进一步提高。体外释放分析表明，CLEO 经历了两阶段的释放机制，在 12 小时内快速释放，然后持续释放。应用的两种合成方法与更高的壳聚糖浓度协同作用，成功地产生了包封效率（EE）大于 60%的纳米结构。这表明这两种技术都可以可靠地保护 CLEO 的生物活性成分，以备进一步使用。
