Hartman Kacper, Świerczyńska Maja, Wieczorek Amelia, Baszuk Piotr, Wojciechowska-Koszko Iwona, Garbacz Katarzyna, Sienkiewicz Monika, Kwiatkowski Paweł
Department of Diagnostic Immunology, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland.
Department of Genetics and Pathology, International Hereditary Cancer Center, Pomeranian Medical University in Szczecin, 71-252 Szczecin, Poland.
Antibiotics (Basel). 2025 Jul 21;14(7):733. doi: 10.3390/antibiotics14070733.
: Growing antibiotic resistance is one of the most significant problems of current medicine. Various research efforts are focused on the search for new substances and their combinations as potential solutions to this problem. Essential oil compounds (EOCs) are considered promising candidates in this regard. However, the interactions between these natural compounds remain understudied. This study conducted a preliminary evaluation of the antimicrobial action of various commercial EOCs (1,8-cineole, eugenol, linalyl acetate, (-)-α-pinene, limonene, α-terpineol, DL-menthol, geraniol, farnesol, carvacrol, and myrcene) alone and in combination ( 56) against methicillin-resistant strain (ATCC 43300). : The following parameters were studied: antibacterial activity of EOCs alone and in combination using microdilution and checkerboard assays. : After the initial screening, geraniol, farnesol, linalyl acetate, carvacrol, (-)-α-pinene, α-terpineol, 1,8-cineole, and eugenol exhibited antibacterial activity against the tested strain and were, therefore, selected for further evaluation in the checkerboard assay. The checkerboard assay revealed 10 synergistic interactions, with farnesol demonstrating the highest number of synergistic combinations among the tested compounds. The results highlighted its high synergistic potential in combination with eugenol, linalyl acetate, (-)-α-pinene, α-terpineol, geraniol, and carvacrol. : In conclusion, the results help elucidate the different interactions between EOCs and may be helpful in further applications of natural compounds as antimicrobial agents in wound dressings. Overall, the most promising compound was found to be farnesol.
抗生素耐药性不断增强是当前医学领域最重大的问题之一。各种研究工作都集中在寻找新物质及其组合，作为解决这一问题的潜在方案。在这方面，精油化合物（EOCs）被认为是有前景的候选物。然而，这些天然化合物之间的相互作用仍未得到充分研究。本研究对多种市售EOCs（1,8-桉叶素、丁香酚、乙酸芳樟酯、（-）-α-蒎烯、柠檬烯、α-松油醇、DL-薄荷醇、香叶醇、法尼醇、香芹酚和月桂烯）单独及组合（共56种）对耐甲氧西林菌株（ATCC 43300）的抗菌作用进行了初步评估。研究了以下参数：使用微量稀释法和棋盘法测定EOCs单独及组合的抗菌活性。初步筛选后，香叶醇、法尼醇、乙酸芳樟酯、香芹酚、（-）-α-蒎烯、α-松油醇、1,8-桉叶素和丁香酚对受试菌株表现出抗菌活性，因此被选用于棋盘法的进一步评估。棋盘法揭示了10种协同相互作用，法尼醇在所测试的化合物中表现出最多的协同组合。结果突出了其与丁香酚、乙酸芳樟酯、（-）-α-蒎烯、α-松油醇、香叶醇和香芹酚组合时的高协同潜力。总之，这些结果有助于阐明EOCs之间的不同相互作用，可能有助于天然化合物作为抗菌剂在伤口敷料中的进一步应用。总体而言，最有前景的化合物是法尼醇。
