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澳大利亚眼镜蛇科毒蛇(棕伊澳蛇,Pseudechis australis)毒液中磷脂酶A2的氨基酸序列。

Amino acid sequences of phospholipases A2 from the venom of an Australian elapid snake (king brown snake, Pseudechis australis).

作者信息

Nishida S, Terashima M, Tamiya N

出版信息

Toxicon. 1985;23(1):87-104. doi: 10.1016/0041-0101(85)90112-6.

DOI:10.1016/0041-0101(85)90112-6
PMID:3887651
Abstract

Two basic phospholipases A2 (Pa-11 and Pa-13) have been isolated from the venom of an Australian elapid snake, Pseudechis australis (king brown snake). The reduced and S-carboxymethylated phospholipases A2 were digested with trypsin and the resulting peptides were purified by a combination of chromatography on a DEAE-cellulose DE-52 column and gel filtration procedures. Eleven main peptides from Pa-11 and 9 peptides from Pa-13 could account for the amino acid compositions of the respective enzyme molecules. The alignment of the tryptic peptides and unelucidated regions of the amino acid sequences of tryptic peptides were established by the analysis of the peptides obtained by chymotryptic and/or Staphylococcal protease digestions. Each phospholipase A2 consisted of a single chain of 118 amino acid residues, including 14 half-cystine residues. Although Pa-11 is enzymatically 30-times as active as Pa-13 and highly toxic as compared to Pa-13, they are highly homologous in their amino acid sequences. They are also homologous to the enzymes from mammalian pancreas and the other snake venom phospholipases A2, especially to those from snakes belonging to the subfamilies Acanthophiinae and Laticaudinae.

摘要

从澳大利亚眼镜蛇科蛇(棕伊澳蛇)的毒液中分离出了两种基本的磷脂酶A2(Pa - 11和Pa - 13)。将还原和S - 羧甲基化的磷脂酶A2用胰蛋白酶消化,所得肽段通过在DEAE - 纤维素DE - 52柱上的色谱法和凝胶过滤程序相结合进行纯化。来自Pa - 11的11个主要肽段和来自Pa -

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引用本文的文献

1
Isolation and amino acid sequence of a short-chain neurotoxin from an Australian elapid snake, Pseudechis australis.从澳大利亚眼镜蛇(澳太攀蛇)中分离出一种短链神经毒素及其氨基酸序列。
Biochem J. 1985 Dec 1;232(2):367-71. doi: 10.1042/bj2320367.
2
Isolation, properties and amino acid sequences of a phospholipase A2 and its homologue without activity from the venom of a sea snake, Laticauda colubrina, from the Solomon Islands.从所罗门群岛海蛇(Laticauda colubrina)毒液中分离出一种磷脂酶A2及其无活性同源物,研究其性质和氨基酸序列。
Biochem J. 1988 Aug 1;253(3):869-75. doi: 10.1042/bj2530869.