从澳大利亚眼镜蛇（澳太攀蛇）中分离出一种短链神经毒素及其氨基酸序列。
Isolation and amino acid sequence of a short-chain neurotoxin from an Australian elapid snake, Pseudechis australis.
作者信息
Takasaki C, Tamiya N
出版信息
Biochem J. 1985 Dec 1;232(2):367-71. doi: 10.1042/bj2320367.
Abstract
A short-chain neurotoxin Pseudechis australis a (toxin Pa a) was isolated from the venom of an Australian elapid snake Pseudechis australis (king brown snake) by sequential chromatography on CM-cellulose, Sephadex G-50 and CM-cellulose columns. Toxin Pa a has an LD50 (intravenous) value of 76 micrograms/kg body wt. in mice and consists of 62 amino acid residues. The amino acid sequence of Pa a shows considerable homology with those of short-chain neurotoxins of elapid snakes, especially of true sea snakes.
摘要
通过在CM-纤维素、葡聚糖G-50和CM-纤维素柱上进行连续色谱法，从澳大利亚眼镜蛇科蛇（棕伊澳蛇）的毒液中分离出一种短链神经毒素——澳大利亚拟眼镜蛇毒素a（毒素Pa a）。毒素Pa a对小鼠的半数致死剂量（静脉注射）为76微克/千克体重，由62个氨基酸残基组成。Pa a的氨基酸序列与眼镜蛇科蛇尤其是真海蛇的短链神经毒素的氨基酸序列具有相当高的同源性。
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