Institute of Modern Optics, Tianjin Key Laboratory of Micro-scale Optical Information Science and Technology, Nankai University, Tianjin, China.
Department of Oral Pathology, Tianjin Stomatological Hospital, Hospital of Stomatology, Nankai University, Tianjin, China.
J Biophotonics. 2024 Jul;17(7):e202400028. doi: 10.1002/jbio.202400028. Epub 2024 Jun 14.
Skin burns that include tissue coagulation necrosis imply variations in stiffness. Dynamic phase-sensitive optical coherence elastography (OCE) is used to evaluate the stiffness of burned skin nondestructively in this paper. The homemade dynamic OCE was initially verified through tissue-mimicking phantom experiments regarding Rayleigh wave speed. After being burned with a series of temperatures and durations, the corresponding structure and stiffness variations of mice skin were demonstrated by histological images, optical coherence tomography B-scans, and OCE elastic wave speed maps. The results clearly displayed the variation in elastic properties and stiffness of the scab edge extending in the lateral direction. Statistical analysis revealed that murine skin burned at temperatures exceeding 100°C typically exhibited greater stiffness than skin burned at temperatures below 100°C. The dynamic OCE technique shows potential application for incorporating elasticity properties as a biomechanical extension module to diagnose skin burn injuries.
皮肤烧伤包括组织凝固性坏死意味着硬度的变化。本文采用动态相敏光相干弹性成像（OCE）技术对烧伤皮肤的硬度进行非破坏性评估。通过组织模拟体实验对自制的动态 OCE 进行了初步验证，检测了瑞利波速度。对一系列温度和持续时间进行烧伤后，通过组织学图像、光相干断层扫描 B 扫描和 OCE 弹性波速度图显示了小鼠皮肤的相应结构和硬度变化。结果清楚地显示了沿侧向扩展的痂边缘的弹性特性和硬度的变化。统计分析表明，温度超过 100°C 的皮肤烧伤通常比温度低于 100°C 的皮肤烧伤具有更大的硬度。动态 OCE 技术有望作为生物力学扩展模块将弹性特性纳入其中，用于诊断皮肤烧伤损伤。
