Pedal I, Baedeker C
Z Rechtsmed. 1985;94(1):9-20. doi: 10.1007/BF00200519.
In contrast to blood serology, which usually fails in specimens more than a few days old, immunohistochemistry (PAP technique) provided reliable information on the blood group (ABO) and, in most cases, also the secretor character of 23 kidney specimens stored for months at room temperature. Better results were obtained with monoclonal antibodies than with human sera. In the late stages of decomposition, blood group diagnosis is based on the more decomposition-resistant antigens of the collecting tubular epithelium (in secretors) and the endothelia of the arteriolae medullares rectae and not on the identification of erythrocytic antigens. In addition, a decomposition-resistant epithelial antigen in the distal convoluted tubules (Tc II) is unmasked by autolysis or heterolysis. "Blood group" antigens were frequently detected in bacteria and fungi. These antigens, however, were clearly distinguishable from blood group characters of the tissue. A transient, weak, false-positive reaction with monoclonal anti-B appeared in decomposed Tc II epithelia.
与血液血清学不同，血液血清学通常在几天以上的陈旧标本中失效，免疫组织化学（PAP技术）能提供有关血型（ABO）的可靠信息，并且在大多数情况下，还能提供23份在室温下储存数月的肾脏标本的分泌型特征。使用单克隆抗体比使用人血清获得了更好的结果。在分解后期，血型诊断基于集合管上皮（分泌型）以及直小动脉内皮中更抗分解的抗原，而不是基于红细胞抗原的鉴定。此外，远曲小管中的一种抗分解上皮抗原（Tc II）会因自溶或异溶而暴露。在细菌和真菌中经常检测到“血型”抗原。然而，这些抗原与组织的血型特征明显不同。在分解的Tc II上皮中出现了与单克隆抗B的短暂、微弱的假阳性反应。
