School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan, ROC.
Department of Pediatrics, Taipei Medical University Hospital, Taipei 11031, Taiwan, ROC.
J Trace Elem Med Biol. 2024 Sep;85:127479. doi: 10.1016/j.jtemb.2024.127479. Epub 2024 Jun 2.
Recent studies indicated that bioactive lipids of phosphatidylcholines (PCs) and lysophosphatidylcholines (LysoPCs) predict unhealthy metabolic phenotypes, but results remain inconsistent. To fill this knowledge gap, we investigated whether essential trace elements affect PC-Lyso PC remodeling pathways and the risk of insulin resistance (IR).
Anthropometric and blood biochemical data (glucose, insulin, and lipoprotein-associated phospholipase A2 (Lp-PLA2)) were obtained from 99 adults. Blood essential/probably essential trace elements and lipid metabolites were respectively measured by inductively coupled plasma mass spectrometry (ICP-MS), and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS).
Except for LysoPC (O-18:0/0:0), an inverse V shape was observed between body weight and PC and LysoPC species. A Pearson correlation analysis showed that essential/probably-essential metals (Se, Cu, and Ni: r=-0.4∼-0.7) were negatively correlated with PC metabolites but positively correlated with LysoPC (O-18:0/0:0) (Se, Cu, and Ni: r=0.85-0.64). Quantile-g computation showed that one quantile increase in essential metals was associated with a 2.16-fold increase in serum Lp-PLA2 (β=2.16 (95 % confidence interval (CI): 0.34, 3.98), p=0.023), which are key enzymes involved in PC/Lyso PC metabolism. An interactive analysis showed that compared to those with the lowest levels (reference), individuals with the highest levels of serum PCs (pooled, M2) and the lowest essential/probably essential metals (M1) were associated with a healthier body composition and had a 76 % decreased risk of IR (odds ratio (OR)=0.24 (95 % CI: 0.06, 0.90), p<0.05). In contrast, increased exposure to LysoPC(O-18:0/0:0) (M2) and essential metals (M2) exhibited an 8.22-times highest risk of IR (OR= 8.22 (2.07, 32.57), p<0.05) as well as an altered body composition. In conclusion, overexposure to essential/probably essential trace elements may promote an unhealthy body weight and IR through modulating PC/LysoPC remodeling pathways.
最近的研究表明，磷脂酰胆碱（PCs）和溶血磷脂酰胆碱（LysoPCs）的生物活性脂质可以预测不健康的代谢表型，但结果仍不一致。为了填补这一知识空白，我们研究了必需微量元素是否会影响 PC-Lyso PC 重塑途径和胰岛素抵抗（IR）的风险。
从 99 名成年人中获取人体测量和血液生化数据（血糖、胰岛素和脂蛋白相关磷脂酶 A2（Lp-PLA2））。通过电感耦合等离子体质谱法（ICP-MS）和超高效液相色谱-质谱法（UPLC-MS）分别测量血液必需/可能必需微量元素和脂质代谢物。
除了 LysoPC（O-18：0/0：0）之外，体重与 PC 和 LysoPC 种类之间呈反“V”形关系。Pearson 相关分析表明，必需/可能必需金属（硒、铜和镍：r=-0.4∼-0.7）与 PC 代谢物呈负相关，但与 LysoPC（O-18：0/0：0）呈正相关（硒、铜和镍：r=0.85-0.64）。分位数计算表明，必需金属的一个分位数增加与血清 Lp-PLA2 增加 2.16 倍相关（β=2.16（95%置信区间：0.34，3.98），p=0.023），Lp-PLA2 是参与 PC/Lyso PC 代谢的关键酶。交互分析表明，与最低水平（参考）相比，血清 PCs 最高水平（合并，M2）和必需/可能必需金属最低水平（M1）的个体具有更健康的身体成分，IR 的风险降低了 76%（比值比（OR）=0.24（95%置信区间：0.06，0.90），p<0.05）。相反，暴露于 LysoPC（O-18：0/0：0）（M2）和必需金属（M2）增加与 IR 的最高 8.22 倍风险相关（OR=8.22（2.07，32.57），p<0.05）以及身体成分的改变。总之，过度暴露于必需/可能必需微量元素可能通过调节 PC/Lyso PC 重塑途径促进不健康的体重和 IR。
