College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, China; Hebi College of Engineering and Technology, Henan Polytechnic University, China.
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, China.
Pestic Biochem Physiol. 2024 Jun;202:105958. doi: 10.1016/j.pestbp.2024.105958. Epub 2024 May 16.
The wheat aphid Sitobion miscanthi is a dominant and destructive pest in agricultural production. Insecticides are the main substances used for effective control of wheat aphids. However, their extensive application has caused severe resistance of wheat aphids to some insecticides; therefore, exploring resistance mechanisms is essential for wheat aphid management. In the present study, CYP6CY2, a new P450 gene, was isolated and overexpressed in the imidacloprid-resistant strain (SM-R) compared to the imidacloprid-susceptible strain (SM-S). The increased sensitivity of S. miscanthi to imidacloprid after knockdown of CYP6CY2 indicates that it could be associated with imidacloprid resistance. Subsequently, the posttranscriptional regulation of CYP6CY2 in the 3' UTR by miR-3037 was confirmed, and CYP6CY2 participated in imidacloprid resistance. This finding is critical for determining the role of P450 in relation to the resistance of S. miscanthi to imidacloprid. It is of great significance to understand this regulatory mechanism of P450 expression in the resistance of S. miscanthi to neonicotinoids.
麦长管蚜 Sitobion miscanthi 是农业生产中一种占优势且具破坏性的害虫。杀虫剂是有效控制麦长管蚜的主要物质。然而，它们的广泛应用导致了麦长管蚜对一些杀虫剂的严重抗性；因此，探索抗性机制对于麦长管蚜的管理至关重要。本研究中，与敏感品系（SM-S）相比，在抗吡虫啉品系（SM-R）中分离并过表达了一个新的 P450 基因 CYP6CY2。CYP6CY2 敲低后，麦长管蚜对吡虫啉的敏感性增加，表明其可能与吡虫啉抗性有关。随后，通过 miR-3037 对 CYP6CY2 的 3'UTR 的转录后调控得到了证实，CYP6CY2 参与了吡虫啉抗性。这一发现对于确定 P450 在麦长管蚜对吡虫啉抗性中的作用具有重要意义。了解 P450 表达在麦长管蚜对新烟碱类杀虫剂抗性中的这种调控机制具有重要意义。
