Johnson S W, Hoffer B J, Baker R, Freedman R
Alcohol Clin Exp Res. 1985 Jan-Feb;9(1):56-8. doi: 10.1111/j.1530-0277.1985.tb05050.x.
The effect of locally applied ethanol on spontaneous discharge rates of cerebellar Purkinje neurons was compared in two inbred strains of rats which differ in their sensitivities to the acute hypnotic effects of this agent. Ethanol, applied locally to neurons by micropressure ejection with multibarrel micropipettes, was significantly more potent for reducing neuronal firing rates in Fischer 344 rats when compared to Brown Norway rats. The hypnotic effect of ethanol, measured by loss of righting reflex, lasted significantly longer in the Fischer 344 strain of rats. These results suggest that susceptibility to a behavioral effect of ethanol might correlate with an effect on firing rates of cerebellar Purkinje cells in rats. In mice, selective breeding has produced "long-sleep" and "short-sleep" strains of mice which differ not only in sensitivity to the hypnotic effect of ethanol, but also to the sensitivity of cerebellar Purkinje cells to depressant effects of this drug. Because electrophysiological investigations are in many ways easier to perform in rats than in mice, genetic differences in rats may provide an important means for analyzing central nervous system actions of acute ethanol administration.
比较了局部应用乙醇对两种近交系大鼠小脑浦肯野神经元自发放电率的影响，这两种大鼠对该药物的急性催眠作用敏感性不同。通过多管微量移液器微量加压喷射将乙醇局部应用于神经元，与布朗挪威大鼠相比，在费希尔344大鼠中，乙醇在降低神经元放电率方面的效力显著更强。通过翻正反射消失来衡量，乙醇的催眠作用在费希尔344品系大鼠中持续的时间显著更长。这些结果表明，大鼠对乙醇行为效应的易感性可能与对小脑浦肯野细胞放电率的影响相关。在小鼠中，选择性育种产生了“长睡眠”和“短睡眠”品系的小鼠，它们不仅对乙醇的催眠作用敏感性不同，而且小脑浦肯野细胞对该药物抑制作用的敏感性也不同。由于在许多方面，电生理研究在大鼠中比在小鼠中更容易进行，大鼠的基因差异可能为分析急性乙醇给药对中枢神经系统的作用提供重要手段。
