采用pH计法进行赖氨酸脱羧酶测定。
Lysine decarboxylase assay by the pH-stat method.
作者信息
Vienozinskiene J, Januseviciute R, Pauliukonis A, Kazlauskas D
出版信息
Anal Biochem. 1985 Apr;146(1):180-3. doi: 10.1016/0003-2697(85)90413-0.
PMID:3887985
Abstract
A simple assay of lysine decarboxylase is described. It is based on measuring the rate of titration of OH- ions, released during decarboxylation with the aid of a pH-stat apparatus. The continuous recording of the reaction progress may be easily performed. Using the pH-stat method the Km for lysine decarboxylase from E. coli is 2.0 mM.
摘要
本文描述了一种简单的赖氨酸脱羧酶检测方法。该方法基于借助pH计测量脱羧过程中释放的OH-离子的滴定速率。反应进程的连续记录可轻松完成。使用pH计法测得大肠杆菌赖氨酸脱羧酶的Km值为2.0 mM。
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