Shi Dongjie, Yang Bintong, Zhao Tong, Sun Wenchao, Cui Haiying, Kang Yuanhuan
Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Fisheries Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
College of Veterinary Medicine/College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
J Fish Dis. 2024 Sep;47(9):e13986. doi: 10.1111/jfd.13986. Epub 2024 Jun 16.
Aeromonas veronii is an important pathogen found in various aquatic environments and products, posing a threat to public health. The Hanks-like serine/threonine protein kinase is closely linked to the pathogenesis of pathogenic bacteria, but the exact role of YihE in A. veronii remains still unknown. To study the specific function of the YihE kinase, we constructed a knockout mutant of the yihE gene in A. veronii. The deletion of the yihE gene resulted in changes to the metabolism of L-arginine-AMC and acetic acid, as well as enhanced resistance to ampicillin and kanamycin in A. veronii. Additionally, the ΔyihE strain demonstrated a 1.4-fold increase in biofilm formation ability and a 1.8-fold decrease in adhesion and invasion to EPCs when compared to the wild-type strain. A significant decrease in cytotoxicity was observed at 2 and 3 h post-infection with EPCs compared to the wild-type strain. Additionally, the deletion of the yihE gene was associated with a significant decrease in motility of the strain. Furthermore, the deletion of the yihE gene resulted in a 1.44-fold increase in the LD of A. veronii in zebrafish. These findings offer valuable insights into the pathogenic mechanisms of A. veronii.
维氏气单胞菌是一种在各种水生环境和产品中发现的重要病原体，对公众健康构成威胁。类汉克斯丝氨酸/苏氨酸蛋白激酶与病原菌的发病机制密切相关，但YihE在维氏气单胞菌中的具体作用仍不清楚。为了研究YihE激酶的特定功能，我们构建了维氏气单胞菌yihE基因的敲除突变体。yihE基因的缺失导致维氏气单胞菌中L-精氨酸-AMC和乙酸的代谢发生变化，以及对氨苄青霉素和卡那霉素的抗性增强。此外，与野生型菌株相比，ΔyihE菌株的生物膜形成能力增加了1.4倍，对EPCs的粘附和侵袭能力降低了1.8倍。与野生型菌株相比，在感染EPCs后2小时和3小时观察到细胞毒性显著降低。此外，yihE基因的缺失与该菌株的运动性显著降低有关。此外，yihE基因的缺失导致维氏气单胞菌在斑马鱼中的半数致死剂量增加了1.44倍。这些发现为维氏气单胞菌的致病机制提供了有价值的见解。
